Serum free light chains in a racially diverse population including African Americans and populations from South Africa

L Luca Bertamini J Jean-Baptiste Alberge D David J. Lee H Habib El-Khoury S Sungjae Kim (1Dana-Farber Cancer Institute, Boston, United States) G Grace Fleming (1Dana-Farber Cancer Institute, Boston, United States) C Ciara Murphy (1Dana-Farber Cancer Institute, Boston, United States) J Julia Colchie (1Dana-Farber Cancer Institute, Boston, United States) M Maya I. Davis (1Center for Early Detection and Interception of Blood Cancers, Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA) J Jacqueline Perry E Elizabeth D. Lightbody S Sabine Allam (1Dana-Farber Cancer Institute, Boston, United States) L Lindokuhle N. Goqwana (5Division of Internal Medicine, Faculty of Health Sciences, School of Clinical Medicine, University of the Witwatersrand, Johannesburg, South Africa) V Vinitha Philip (6Clinical Haematology Unit, Department of Medicine, Faculty of Health Sciences, Chris Hani Baragwanath Academic Hospital, University of the Witwatersrand, Johannesburg, South Africa) N Natalie Smyth D Dhananjay Sakrikar (9The Binding Site, a part of ThermoFisher Scientific, Boston, United States) M Mark Perkins (9Binding Site Group, Part of Thermo Fisher Scientific, Birmingham, United Kingdom) S Stephen Harding (2The Binding Site (part of Thermo Fisher Scientific), Birmingham, United Kingdom) D Derek Troske (9The Binding Site, a part of ThermoFisher Scientific, Boston, United States) G Gad Getz E Elizabeth W. Karlson N Nikhil Munshi (3VA Boston Healthcare System, Boston, MA) K Kenneth C. Anderson L Lorenzo Trippa (From Médecins Sans Frontières (L.G., F.V.), Sorbonne Université, INSERM Unité 1135, Centre d’Immunologie et des Maladies Infectieuses (L.G.), Assistance Publique–Hôpitaux de Paris, Groupe Hospitalier Universitaire Sorbonne Université, Hôpital Pitié–Salpêtrière, Centre National de Référence des Mycobactéries et de la Résistance des Mycobactéries aux Antituberculeux (L.G.), and Epicentre (M.G., E. Baudin), Paris, and Translational Research on HIV and Endemic and Emerging Infectious Diseases, Montpellier Université de Montpellier, Montpellier, Institut de Recherche pour le Développement, Montpellier, INSERM, Montpellier (M.B.) — all in France; Interactive Development and Research, Singapore (U.K.); McGill University, Epidemiology, Biostatistics, and Occupational Health, Montreal (U.K.); UCSF Center for Tuberculosis (G.E.V., P.N., P.P.J.P.) and the Division of HIV, Infectious Diseases, and Global Medicine (G.E.V.), University of California at San Francisco, San Francisco; the National Scientific Center of Phth...) C Catherine R. Marinac (1Center for Early Detection and Interception of Blood Cancers, Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA) W Wenlong C. Chen M Maureen Joffe I Irene M. Ghobrial

Abstract

Abstract Detection of light chain (LC) monoclonal gammopathies (MGs) traditionally relies on serum free LC (FLC) κ, λ, and their ratio (κ/λ) reference ranges based on a mostly White population. We investigated FLC values in a racially diverse population by screening 10 035 individuals for heavy chain MG, identifying 9028 negative cases whose FLC were measured. Participants included 4149 from the PROMISE study (United States, n = 2383; South Africa, n = 1766) and 4879 from the Mass General Brigham Biobank, with 44% self-identifying as Black. Using standard FLC reference ranges, 1074 of 10 035 individuals (10.7%) were diagnosed with LC monoclonal gammopathy of undetermined significance (MGUS), with 99% being κ-restricted. In the United States, 14.8% of Black and 4% of White individuals were diagnosed (P < .01). Among US participants of African (AFR) and European (EUR) genetic ancestry, 14.4% AFR and 2.9% EUR were diagnosed (P < .01). Among South Africans (100% Black), 27.8% were diagnosed using standard ranges. To avoid overdiagnosis, we propose a new κ/λ ratio reference range (0.686 to 2.10) for populations of AFR descent with normal renal function, with standard values for κ and λ being 7.97 to 77.50 mg/L and 6.20 to 49.20 mg/L, respectively. This reduces LC-MGUS overdiagnosis by 91% (10.7% vs 0.97%). Using the new reference, LC-MGUS accounts for 8.8% of MGUS cases, with 74% being κ-restricted, consistent with LC myeloma rates. These findings highlight the importance of basing disease definitions, such as MGUS, on diverse populations. Adopting our proposed FLC reference values would reduce MGUS overdiagnosis among Black individuals, avoiding unnecessary financial, psychological, and medical consequences. This study includes data from NCT03689595.

Article Details

Journal Blood
Volume / Issue Vol. 145, Issue 8
Published February 20, 2025
Pages 840-849
ISSN 0006-4971
Publisher Elsevier BV

Journal Info

Blood

Elsevier BV

ISSN: 0006-4971 Health Sciences

Authors (28)

L

Luca Bertamini

J

Jean-Baptiste Alberge

D

David J. Lee

H

Habib El-Khoury

S

Sungjae Kim

1Dana-Farber Cancer Institute, Boston, United States

G

Grace Fleming

1Dana-Farber Cancer Institute, Boston, United States

C

Ciara Murphy

1Dana-Farber Cancer Institute, Boston, United States

J

Julia Colchie

1Dana-Farber Cancer Institute, Boston, United States

M

Maya I. Davis

1Center for Early Detection and Interception of Blood Cancers, Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA

J

Jacqueline Perry

E

Elizabeth D. Lightbody

S

Sabine Allam

1Dana-Farber Cancer Institute, Boston, United States

L

Lindokuhle N. Goqwana

5Division of Internal Medicine, Faculty of Health Sciences, School of Clinical Medicine, University of the Witwatersrand, Johannesburg, South Africa

V

Vinitha Philip

6Clinical Haematology Unit, Department of Medicine, Faculty of Health Sciences, Chris Hani Baragwanath Academic Hospital, University of the Witwatersrand, Johannesburg, South Africa

N

Natalie Smyth

D

Dhananjay Sakrikar

9The Binding Site, a part of ThermoFisher Scientific, Boston, United States

M

Mark Perkins

9Binding Site Group, Part of Thermo Fisher Scientific, Birmingham, United Kingdom

S

Stephen Harding

2The Binding Site (part of Thermo Fisher Scientific), Birmingham, United Kingdom

D

Derek Troske

9The Binding Site, a part of ThermoFisher Scientific, Boston, United States

G

Gad Getz

E

Elizabeth W. Karlson

N

Nikhil Munshi

3VA Boston Healthcare System, Boston, MA

K

Kenneth C. Anderson

L

Lorenzo Trippa

From Médecins Sans Frontières (L.G., F.V.), Sorbonne Université, INSERM Unité 1135, Centre d’Immunologie et des Maladies Infectieuses (L.G.), Assistance Publique–Hôpitaux de Paris, Groupe Hospitalier Universitaire Sorbonne Université, Hôpital Pitié–Salpêtrière, Centre National de Référence des Mycobactéries et de la Résistance des Mycobactéries aux Antituberculeux (L.G.), and Epicentre (M.G., E. Baudin), Paris, and Translational Research on HIV and Endemic and Emerging Infectious Diseases, Montpellier Université de Montpellier, Montpellier, Institut de Recherche pour le Développement, Montpellier, INSERM, Montpellier (M.B.) — all in France; Interactive Development and Research, Singapore (U.K.); McGill University, Epidemiology, Biostatistics, and Occupational Health, Montreal (U.K.); UCSF Center for Tuberculosis (G.E.V., P.N., P.P.J.P.) and the Division of HIV, Infectious Diseases, and Global Medicine (G.E.V.), University of California at San Francisco, San Francisco; the National Scientific Center of Phth...

C

Catherine R. Marinac

1Center for Early Detection and Interception of Blood Cancers, Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA

W

Wenlong C. Chen

M

Maureen Joffe

I

Irene M. Ghobrial