Sensitive pathogen DNA detection by a multi-guide RNA Cas12a assay favoring trans- versus cis-cleavage

Z Zhen Huang Z Zhe Song (State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Drug Design and Optimization, and Department of Chemistry) J Jianfeng Zeng X Xuhui Liu M Mutong Fang Z Zhiyuan Wu Y Yao Zhao Y Yanli Chen D Dan Li H Huan Huang (Beijing Synchrotron Radiation Facility, Institute of High Energy Physics) L Liang Fu P Peng Xu B Bo Ning J Jun Chen M Ming Guan (Department of Laboratory Medicine, Huashan Hospital, Fudan University) L Lin Sun C Christopher J. Lyon X Xiao-Yong Fan (Shanghai Public Health Clinical Center and Shanghai Institute of Infectious Diseases and Biosecurity, Fudan University) S Shuihua Lu T Tony Hu