Selective Leaflet‐Anchored DNA Nanoprobes for Simultaneous Monitoring of Juxta‐Plasma Membrane Environments
Abstract
Abstract The cell membrane functions as a bidirectional interface that coordinates the selective transport of substances and information between the interior and exterior of the cell. Simultaneous monitoring of both the inner and outer local environments surrounding this lipid bilayer is crucial for elucidating various cellular activities but significantly challenged by the lack of technologies capable of precisely engineering biosensing probes on both membrane leaflets. In this work, by developing fusogenic nanoliposomes with high cell fusion efficiency, we successfully anchored amphiphilic DNA tetrahedral probes onto the inner leaflet of the plasma membrane. By integrating this with the direct anchoring of amphiphilic probes on the outer leaflet, we achieved precise functionalization of both leaflets of the cell membrane, thus enabling simultaneous monitoring of localized targets within their respective juxta‐plasma membrane environments, avoiding signal contamination caused by the optical diffraction limit. Using selectively dual‐leaflet‐anchored tetrahedral DNAzyme probes, we revealed that the transmembrane ion channel SLC41A1 synergistically modulated the influx of Na + and the efflux of Mg 2+ in live cells. With a modular design, this membrane‐anchored DNA nanoplatform can be readily extended for the study of bilateral interface‐dominant cellular processes, shifting the paradigm toward a more localized and subtle perspective.
Article Details
Authors (13)
Yao He
Jie Lin
Department of Oncology The Second Affiliated Hospital of Kunming Medical University Kunming China
Fandi Wu
Yong‐Hao Ma
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering College of Biology Aptamer Engineering Center of Hunan Province, Hunan University Changsha Hunan 410082 China
Zhaoyang Wang
Hui Wu
Zhimin Wang
Nachuan Wen
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province
Yutong Zhang
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences
Wenfei Guo
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering College of Biology Aptamer Engineering Center of Hunan Province, Hunan University Changsha Hunan 410082 China
Yulin Du
Weihong Tan
Institute of Molecular Medicine (IMM), Department of Nephrology, Molecular Cell Laboratory for Kidney Disease, Shanghai Peritoneal Dialysis Research Center, Uremia Diagnosis and Treatment Center, State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, School of Chemistry and Chemical Engineering
Liping Qiu
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and Biosensing, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province