Scalable Hyperpolarized MRI Enabled by Ace‐SABRE of [1‐ <sup>13</sup> C]Pyruvate
Abstract
Abstract Hyperpolarized (HP) MRI using [1– 13 C]pyruvate is emerging as a promising molecular imaging approach. Among hyperpolarization methods, Signal Amplification By Reversible Exchange (SABRE) is attractive because SABRE polarizes the substrates directly in room‐temperature solutions avoiding complex hardware. Most SABRE experiments have historically been performed in methanol, a relatively toxic and difficult‐to‐remove solvent. Here we demonstrate the use of a 80/20 acetone/water (A/W) solvent system (Ace‐SABRE) to provide hyperpolarized [1– 13 C]pyruvate with up to 17% polarization, then implement a solvent processing protocol to achieve injectable solutions retaining 74% of the initial polarization, and lastly we demonstrate HP in vivo spectroscopy and imaging using the Ace‐SABRE platform to showcase metabolic tracking in a hepatocellular carcinoma (HCC) tumor as well as HP‐MRI, both in direct comparison to dissolution dynamic nuclear polarization (d‐DNP) experiments. The Ace‐SABRE technique promises faster adoption of SABRE hyperpolarization in biological experiments, overall lowering the barriers to entry for HP‐NMR and HP‐MRI.
Article Details
Authors (22)
Stephen J. McBride
Department of Chemistry North Carolina State University Raleigh NC USA
Megan Pike
Department of Chemistry North Carolina State University Raleigh NC USA
Erica Curran
Department of Chemistry North Carolina State University Raleigh NC USA
Alexander Zavriyev
Department of Radiology University of Pennsylvania Philadelphia PA USA
Bukola Adebesin
Department of Radiology University of Pennsylvania Philadelphia PA USA
Luke Tucker
Vizma Life Sciences Chapel Hill NC 27514 USA
Jared M. Harzan
School of Chemical & Biomolecular Sciences Southern Illinois University Carbondale IL USA
Ishani M. Senanayake
School of Chemical & Biomolecular Sciences Southern Illinois University Carbondale IL USA
Mustapha Abdulmojeed
Department of Chemistry North Carolina State University Raleigh NC USA
Franziska Theiss
Department of Chemistry North Carolina State University Raleigh NC USA
Sheng Shen
Thomas Boele
Simon B. Duckett
Department of Chemistry
Boyd M. Goodson
School of Chemical & Biomolecular Sciences and Materials Technology Center
Matthew S. Rosen
Eduard Y. Chekmenev
Department of Chemistry, Integrative Biosciences (Ibio)
Hong Yuan
Clinical Laboratory Center, Central Hospital of Dalian University of Technology
Carlos Dedesma
Vizma Life Sciences Chapel Hill NC 27514 USA
Terence Gade
Department of Radiology University of Pennsylvania Philadelphia PA USA
Stephen Kadlecek
Department of Radiology University of Pennsylvania Philadelphia PA USA
Thomas Theis
Department of Chemistry Department of Physics Comparative Medicine Institute North Carolina State University Raleigh North Carolina 27695–8204, 513 USA
Patrick TomHon
Vizma Life Sciences Chapel Hill NC 27514 USA