Reduced Cas9 transgene silencing by incorporation of intron sequences

Sophia Arana; Peter P. Du; Alun Vaughan-Jackson; Nora Enright; Kaitlyn Spees; Raeline Valbuena; Cesar A. Garcia; Thien Nguyen; Anthony Venida; Marta Seczynska; Lacramioara Bintu; Paul J. Lehner; Laura M. Prolo; Michael C. Bassik

doi:10.1038/s41467-025-65669-0

Reduced Cas9 transgene silencing by incorporation of intron sequences

S Sophia Arana P Peter P. Du A Alun Vaughan-Jackson N Nora Enright K Kaitlyn Spees R Raeline Valbuena C Cesar A. Garcia (Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Dr. East, Los Angeles, California 90095, United States) T Thien Nguyen A Anthony Venida M Marta Seczynska L Lacramioara Bintu P Paul J. Lehner (Cambridge Institute for Therapeutic Immunology and Infectious Disease, Cambridge University) L Laura M. Prolo M Michael C. Bassik

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Article Details

Journal Nature Communications

Volume / Issue Vol. 16, Issue 1

Published November 27, 2025

DOI 10.1038/s41467-025-65669-0

ISSN 2041-1723

Publisher Nature Portfolio

Journal Info

Nature Communications

Nature Portfolio

ISSN: 2041-1723 Open Access Life Sciences

Authors (14)

S

Sophia Arana

P

Peter P. Du

A

Alun Vaughan-Jackson

N

Nora Enright

K

Kaitlyn Spees

R

Raeline Valbuena

C

Cesar A. Garcia

Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Dr. East, Los Angeles, California 90095, United States

T

Thien Nguyen

A

Anthony Venida

M

Marta Seczynska

L

Lacramioara Bintu

P

Paul J. Lehner

Cambridge Institute for Therapeutic Immunology and Infectious Disease, Cambridge University

L

Laura M. Prolo

M

Michael C. Bassik

Reduced Cas9 transgene silencing by incorporation of intron sequences

Article Details

Journal Info

Authors (14)

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