Red-light signaling pathway activates desert cyanobacteria to prepare for desiccation tolerance

H Hai-Feng Xu (School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University) G Guo-Zheng Dai (School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University) R Ren-Han Li (School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University) Y Yang Bai A Ai-Wei Zuo (School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University) L Lei Zhao (School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University) S Shu-Ren Cui (School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University) J Jin-Long Shang (School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University) C Chao Cheng (School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University) Y Yu-Jie Wang (School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University) G Gui-Fang Feng (School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University) D Deqiang Duanmu (National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University) A Aaron Kaplan (Department of Plant and Environmental Sciences, Edmond J. Safra Campus, The Hebrew University of Jerusalem) B Bao-Sheng Qiu (School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University)

Abstract

Desiccation-tolerant cyanobacteria are able to survive frequent cycles of hydration and dehydration, which are closely linked to diurnal light oscillations. Previous studies have shown that light serves as a crucial anticipatory cue, activating desert cyanobacteria to prepare for desiccation. However, the mechanisms underlying their light-coupled desiccation tolerance remain largely unknown. Here, we demonstrate that red-light-induced photosynthetic genes are positively regulated by a LuxR family transcription factor NfSrr1. We further identified the cyanobacteriochrome NfPixJ as interacting with NfSrr1 and functioning as a red light sensor. Phenotypic analysis revealed that the red-light signaling module NfPixJ-NfSrr1 plays a key role in preparing cyanobacteria for desiccation tolerance. This module also regulates the synthesis of protective compatible solutes, suggesting that red light functions as a global regulatory signal for the broader stress response. Phylogenetic analysis indicates that the presence of this red-light signaling pathway, mediated by NfPixJ-NfSrr1 module, correlates with the ability of cyanobacteria to thrive in water-deficit habitats. Overall, our findings uncover a red-light signaling pathway that enhances desiccation tolerance as desert cyanobacteria encounter red light at dawn, before water limitation. These results provide insights into the mechanisms behind light-induced anticipatory stress tolerance in photosynthetic organisms.

Article Details

Volume / Issue Vol. 122, Issue 12
Published March 25, 2025
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (14)

H

Hai-Feng Xu

School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University

G

Guo-Zheng Dai

School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University

R

Ren-Han Li

School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University

Y

Yang Bai

A

Ai-Wei Zuo

School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University

L

Lei Zhao

School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University

S

Shu-Ren Cui

School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University

J

Jin-Long Shang

School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University

C

Chao Cheng

School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University

Y

Yu-Jie Wang

School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University

G

Gui-Fang Feng

School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University

D

Deqiang Duanmu

National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University

A

Aaron Kaplan

Department of Plant and Environmental Sciences, Edmond J. Safra Campus, The Hebrew University of Jerusalem

B

Bao-Sheng Qiu

School of Life Sciences, Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University