Rapid microbial production of long-lived dissolved organic carbon in the global ocean

R Ruanhong Cai (Department of Ocean Science and Center for Ocean Research in Hong Kong and Macau, The Hong Kong University of Science and Technology) O Oliver J. Lechtenfeld (Department of Environmental Analytical Chemistry, Research Group BioGeoOmics, Helmholtz Centre for Environmental Research) A Andrew J. Tanentzap J Julian P. Sachs (School of Oceanography, University of Washington) S Shuh‐Ji Kao (State Key Laboratory of Marine Resource Utilization in South China Sea and School of Marine Science and Engineering, Hainan University) H Helena Osterholz (Marine Chemistry, Leibniz Institute for Baltic Sea Research Warnemünde) Z Zhenwei Yan (Department of Ocean Science and Center for Ocean Research in Hong Kong and Macau, The Hong Kong University of Science and Technology) C Chen Zhao Y Yuanbi Yi (Department of Ocean Science and Center for Ocean Research in Hong Kong and Macau, The Hong Kong University of Science and Technology) P Penghui Li C Chuanlun Zhang (Department of Ocean Science and Engineering, Shenzhen Key Laboratory of Marine Archaea Geo-Omics, Southern University of Science and Technology) N Nianzhi Jiao (Department of Marine Biology and Technology, College of Ocean and Earth Sciences and State Key Laboratory of Marine Environmental Science, Xiamen University) B Boris P. Koch (Alfred-Wegener-Institut Helmholtz Zentrum für Polar- und Meeresforschung) G Gerhard J. Herndl (Department of Functional and Evolutionary Ecology, University of Vienna) D Ding He (Department of Ocean Science and Center for Ocean Research in Hong Kong and Macau, The Hong Kong University of Science and Technology)

Abstract

Marine microbes have long been regarded as central to replenishing the ocean’s reservoir of recalcitrant dissolved organic matter (RDOM). However, molecular-level evidence for their role remains inconclusive because RDOM persists for years to millennia, far exceeding timescales accessible to laboratory experiments, and because conventional analytical approaches lack the resolution to discern structural isomers of RDOM that confer functionally important differences in persistence. Using polarity-based liquid chromatography coupled to ultrahigh-resolution mass spectrometry capable of discriminating RDOM isomer clusters, we reveal that marine microbial consortia rapidly (≤ 90 d) convert diverse organic substrates into RDOM with extensive structural isomerism that closely mirrors natural seawater RDOM. A subset of these microbially derived RDOM compounds exhibits near-ubiquitous occurrence (> 99%) in a global dataset and accumulates progressively in the ocean’s interior. Together, our findings substantiate the direct microbial contribution to the long-lived oceanic carbon reservoir through the rapid diversification of RDOM isomers, a mechanism that contributes to sustaining the complexity and long-term persistence of the planetary-scale carbon reservoir.

Article Details

Volume / Issue Vol. 123, Issue 31
Published August 04, 2026
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (15)

R

Ruanhong Cai

Department of Ocean Science and Center for Ocean Research in Hong Kong and Macau, The Hong Kong University of Science and Technology

O

Oliver J. Lechtenfeld

Department of Environmental Analytical Chemistry, Research Group BioGeoOmics, Helmholtz Centre for Environmental Research

A

Andrew J. Tanentzap

J

Julian P. Sachs

School of Oceanography, University of Washington

S

Shuh‐Ji Kao

State Key Laboratory of Marine Resource Utilization in South China Sea and School of Marine Science and Engineering, Hainan University

H

Helena Osterholz

Marine Chemistry, Leibniz Institute for Baltic Sea Research Warnemünde

Z

Zhenwei Yan

Department of Ocean Science and Center for Ocean Research in Hong Kong and Macau, The Hong Kong University of Science and Technology

C

Chen Zhao

Y

Yuanbi Yi

Department of Ocean Science and Center for Ocean Research in Hong Kong and Macau, The Hong Kong University of Science and Technology

P

Penghui Li

C

Chuanlun Zhang

Department of Ocean Science and Engineering, Shenzhen Key Laboratory of Marine Archaea Geo-Omics, Southern University of Science and Technology

N

Nianzhi Jiao

Department of Marine Biology and Technology, College of Ocean and Earth Sciences and State Key Laboratory of Marine Environmental Science, Xiamen University

B

Boris P. Koch

Alfred-Wegener-Institut Helmholtz Zentrum für Polar- und Meeresforschung

G

Gerhard J. Herndl

Department of Functional and Evolutionary Ecology, University of Vienna

D

Ding He

Department of Ocean Science and Center for Ocean Research in Hong Kong and Macau, The Hong Kong University of Science and Technology