Quantitative Super‐Resolution Imaging of On‐Origami DNA Conformation and Reactivity Under Electric Fields
Abstract
Abstract Self‐assembled DNA nanostructures have been popularly used to develop DNA‐based electrochemical sensors by exploiting the nanoscale positioning capability of DNA origami. However, the impact of the electric field on the structural stability of the DNA origami framework and the activity of carried DNA probes remains to be explored. Herein, we employ DNA origami as structural frameworks for reversible DNA hybridization, and develop a single‐molecule fluorescence imaging method to quantify electric field effects on DNA conformation and hybridization properties at the single‐molecule level. Through single‐molecule temporal kinetic analysis of hybridization events occurring on individual DNA origami, we systematically determine the regulation patterns of applied potential and scanning duration on the activity of DNA probes. Optical super‐resolution reconstruction of probe sites reveals electric field‐induced structural relaxation in DNA frameworks. This approach not only provides insights into electrochemical DNA sensing devices, but also lays the foundation for developing hybrid electrical–optical analysis at the single‐molecule level.
Article Details
Authors (13)
Zhongchao Jin
State Key Laboratory of Synergistic Chem-Bio Synthesis, School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Institute of Translational Medicine, Shanghai Jiao Tong University
Yiyang Zeng
State Key Laboratory of Synergistic Chem‐Bio Synthesis School of Chemistry and Chemical Engineering New Cornerstone Science Laboratory Frontiers Science Center for Transformative Molecules National Center for Translational Medicine Shanghai Jiao Tong University Shanghai 200240 China
Xiaodong Xie
Zheze Dai
State Key Laboratory of Synergistic Chem-Bio Synthesis, School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Institute of Translational Medicine, Shanghai Jiao Tong University
Yao Xie
Jianlei Shen
State Key Laboratory of Synergistic Chem-Bio Synthesis, School of Chemistry and Chemical Engineering, New Cornerstone Science Laboratory, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University
Xiaoguo Liu
State Key Laboratory of Synergistic Chem-Bio Synthesis, School of Chemistry and Chemical Engineering, New Cornerstone Science Laboratory, Frontiers Science Center for Transformative Molecules, Zhangjiang Institute for Advanced Study and National Center for Translational Medicine
Jiang Li
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica
Lihua Wang
Qian Li
Fei Wang
Chunhai Fan
State Key Laboratory of Synergistic Chem-Bio Synthesis, School of Chemistry and Chemical Engineering, New Cornerstone Science Laboratory, Frontiers Science Center for Transformative Molecules, Zhang Jiang Institute for Advanced Study and National Center for Translational Medicine
Hui Lv
Institute of Materiobiology, College of Sciences