Prognostic value of serial liquid biopsy EGFR mutation monitoring in Hispanic patients with advanced NSCLC treated with osimertinib.

J Juan Manuel Garzon-Dangond (Atlantic Health System - Overlook Medical Center, Summit, NJ) A Andrés Felipe Cardona (Arnaud Scherpereel, MD, PhD, CHU Lille, Univ. Lille, Inserm, U1366-UMR9020—CRCLille—Cancer Research Center of Lille, OncoThAI, ONCOLille, Lille, France; Aaron S. Mansfield, MD, Mayo Clinic, Rochester, MN; Francesco Grossi, MD, Medical Oncology Division, Department of Medicine and Technological Innovation, University of Insubria, Varese, Italy; Sanjay Popat, PhD, FRCP, The Royal Marsden Hospital, London, United Kingdom; Paul Baas, MD, PhD, The Netherlands Cancer Institute and Leiden University Medical Center, Amsterdam, the Netherlands; Anna K. Nowak, PhD, MBBS, University of Western Australia, Perth, WA, Australia; Anne S. Tsao, MD, MBA, University of Texas MD Anderson Cancer Center, Houston, TX; Nobukazu Fujimoto, MD, PhD, Okayama Rosai Hospital, Okayama, Japan; Solange Peters, MD, PhD, Lausanne University Hospital, Lausanne, Switzerland; Yolanda Bautista Aragon, MD, Centro Médico Nacional Siglo XXI, Mexico City, Mexico; Toby Talbot, MD, The Sunrise Centre, Royal Cornwall Hospitals NHS Trust, Truro, Unite...) J Jairo Andrés Zuluaga (Luis Carlos Sarmiento Angulo Cancer Treatment and Research Center - CTIC, Bogota, Colombia) L Leonardo Rojas N Nicolle Wagner-Gutiérrez (Universidad de los Andes, Bogota, Colombia) A Alejandro Ruiz-Patiño (Foundation for Clinical and Applied Cancer Research - FICMAC, Bogotá, Colombia) D Diego Chamorro (Fundación para la Investigación Clínica y aplicada del Cancer (FICMAC), Bogotá, Colombia) J July Rodriguez (Fundación para la Investigación Clínica y Molecular Aplicada del Cáncer (FICMAC), Bogotá, Colombia) D Dora Lucia Vallejo-Ardila (Fundación de Investigación Clínica y Molecular Aplicada del Cáncer FICMAC, Bogota, Colombia) L Lucia Viola (Fundacion Neumologica Colombia - Luis Carlos Sarmiento Angulo Cancer Treatment and Research Center, Bogotá, Colombia) S Stella Martinez (Luis Carlos Sarmiento Angulo Cancer Treatment and Research Center (CTIC), Bogotá, Colombia) C Carlos Carvajal (Luis Carlos Sarmiento Angulo Cancer Treatment and Research Center (CTIC), Bogotá, Colombia) P Pilar Archila (Fundación de Investigación Clínica y Molecular Aplicada del Cáncer FICMAC, Bogotá, Colombia) J Juan Esteban Garcia-Robledo (Mayo Clinic Arizona, Scottsdale, AZ) E Elvira Jaller (Thoracic Oncology Unit, National Cancer Institute (INCan), Mexico City, Mexico) C Claudio Martin (Instituto Alexander Fleming, Buenos Aires) L Luis Corrales (Centro de Investigacion y Manejo del Cancer (CIMCA), San José, Costa Rica) C Christian Diego Rolfo (Center for Thoracic Oncology, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY) R Rafael Rosell O Oscar Arrieta

Abstract

e20761 Background: The prognostic role of serial circulating tumor DNA (ctDNA) dynamics during osimertinib treatment has been described mainly in Asian and European populations; however, it remains poorly defined in Hispanic patients with advanced EGFR-mutant non–small cell lung cancer (NSCLC). Our aim was to determine whether serial plasma EGFR testing identifies patients at high risk of early mortality. Methods: This multicenter, retrospective study included 64 Hispanic patients with stage IIIB–IV EGFR-mutant NSCLC treated with first-line osimertinib between 2018 and 2023. Tumor tissue genotyping was performed using hybrid-capture next-generation sequencing. Plasma EGFR mutations were assessed at baseline, week 8, and week 24. Overall survival (OS) and progression-free survival (PFS) were analyzed using Cox models adjusted for p53 status, RAF-pathway alterations, and metastatic burden. Results: The cohort had a median age of 61 years, with 65.6% of patients being female and 56.2% being never-smokers. Sensitizing EGFR mutations consisted mainly of exon 19 deletion (59.4%) and L858R (39.1%). Relevant co-mutations were identified in TP53 (29.7%) and RAF-pathway genes (6.2%). Plasma ctDNA remained positive in 42.2% of patients at week 8 and 22.0% at week 24. Week-8 ctDNA persistence was strongly associated with an inferior OS (HR 9.36, 95% CI 3.80–23.03, p < 0.001) after multivariable adjustment. Week-24 positivity also predicted worse OS (HR 3.34, 95% CI 1.13–9.89, p = 0.02). ctDNA status at week 8 or 24 was not significantly associated with PFS. Patients with TP53 or RAF pathway mutations demonstrated higher rates of molecular persistence. Conclusions: In Hispanic patients receiving Osimertinib, ctDNA positivity at 8-24 weeks is able to identify patients with poor survival outcomes. ctDNA monitoring may provide an early, non-invasive biomarker to guide treatment intensification and trial enrollment in EGFR-mutant NSCLC.

Article Details

Volume / Issue Vol. 44, Issue 16_suppl
Published June 01, 2026
ISSN 0732-183X
Publisher Lippincott Williams & Wilkins

Journal Info

Journal of Clinical Oncology

Lippincott Williams & Wilkins

ISSN: 0732-183X Health Sciences

Authors (20)

J

Juan Manuel Garzon-Dangond

Atlantic Health System - Overlook Medical Center, Summit, NJ

A

Andrés Felipe Cardona

Arnaud Scherpereel, MD, PhD, CHU Lille, Univ. Lille, Inserm, U1366-UMR9020—CRCLille—Cancer Research Center of Lille, OncoThAI, ONCOLille, Lille, France; Aaron S. Mansfield, MD, Mayo Clinic, Rochester, MN; Francesco Grossi, MD, Medical Oncology Division, Department of Medicine and Technological Innovation, University of Insubria, Varese, Italy; Sanjay Popat, PhD, FRCP, The Royal Marsden Hospital, London, United Kingdom; Paul Baas, MD, PhD, The Netherlands Cancer Institute and Leiden University Medical Center, Amsterdam, the Netherlands; Anna K. Nowak, PhD, MBBS, University of Western Australia, Perth, WA, Australia; Anne S. Tsao, MD, MBA, University of Texas MD Anderson Cancer Center, Houston, TX; Nobukazu Fujimoto, MD, PhD, Okayama Rosai Hospital, Okayama, Japan; Solange Peters, MD, PhD, Lausanne University Hospital, Lausanne, Switzerland; Yolanda Bautista Aragon, MD, Centro Médico Nacional Siglo XXI, Mexico City, Mexico; Toby Talbot, MD, The Sunrise Centre, Royal Cornwall Hospitals NHS Trust, Truro, Unite...

J

Jairo Andrés Zuluaga

Luis Carlos Sarmiento Angulo Cancer Treatment and Research Center - CTIC, Bogota, Colombia

L

Leonardo Rojas

N

Nicolle Wagner-Gutiérrez

Universidad de los Andes, Bogota, Colombia

A

Alejandro Ruiz-Patiño

Foundation for Clinical and Applied Cancer Research - FICMAC, Bogotá, Colombia

D

Diego Chamorro

Fundación para la Investigación Clínica y aplicada del Cancer (FICMAC), Bogotá, Colombia

J

July Rodriguez

Fundación para la Investigación Clínica y Molecular Aplicada del Cáncer (FICMAC), Bogotá, Colombia

D

Dora Lucia Vallejo-Ardila

Fundación de Investigación Clínica y Molecular Aplicada del Cáncer FICMAC, Bogota, Colombia

L

Lucia Viola

Fundacion Neumologica Colombia - Luis Carlos Sarmiento Angulo Cancer Treatment and Research Center, Bogotá, Colombia

S

Stella Martinez

Luis Carlos Sarmiento Angulo Cancer Treatment and Research Center (CTIC), Bogotá, Colombia

C

Carlos Carvajal

Luis Carlos Sarmiento Angulo Cancer Treatment and Research Center (CTIC), Bogotá, Colombia

P

Pilar Archila

Fundación de Investigación Clínica y Molecular Aplicada del Cáncer FICMAC, Bogotá, Colombia

J

Juan Esteban Garcia-Robledo

Mayo Clinic Arizona, Scottsdale, AZ

E

Elvira Jaller

Thoracic Oncology Unit, National Cancer Institute (INCan), Mexico City, Mexico

C

Claudio Martin

Instituto Alexander Fleming, Buenos Aires

L

Luis Corrales

Centro de Investigacion y Manejo del Cancer (CIMCA), San José, Costa Rica

C

Christian Diego Rolfo

Center for Thoracic Oncology, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY

R

Rafael Rosell

O

Oscar Arrieta