Phylogenomics redefines the evolutionary history of mosquitoes

M Mac P. Pierce (State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong) B Brian M. Worthington (State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong) S Shaolin Han (Centre for Immunology and Infection Limited) X Xue-Bing Ni (State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong) Y Yunshi Liao (State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong) M Marcus H. Shum (State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong) Y Yi Guan (State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong) E Edward C. Holmes T Tommy T. Lam (State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong)

Abstract

Mosquitoes have a substantial impact on human and animal health, but their deeper evolutionary relationships have been difficult to resolve. We inferred a time-calibrated phylogenetic history of mosquitoes using conserved genome-wide markers from representatives of major lineages. Our analyses revealed that codon bias and positive selection in subfamily Anophelinae contributed to a substantial level of branch attraction bias between Anophelinae and outgroup taxa, which in our view has misled previous phylogenetic analyses of mosquitoes. Accounting for this systematic phylogenetic bias led to a revised view of mosquito evolution, including the nonmonophyly of subfamily Culicinae. Similarly, we dated the origin of mosquitoes to the mid-Cretaceous (~106 Mya) and most extant genera to after the KPg boundary <66 Mya, 100 My younger than previous estimates and coincident with the origin of Plasmodium parasites. Our study provides a foundation for future analyses of the evolution of mosquito-borne disease.

Article Details

Volume / Issue Vol. 122, Issue 42
Published October 21, 2025
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (9)

M

Mac P. Pierce

State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong

B

Brian M. Worthington

State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong

S

Shaolin Han

Centre for Immunology and Infection Limited

X

Xue-Bing Ni

State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong

Y

Yunshi Liao

State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong

M

Marcus H. Shum

State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong

Y

Yi Guan

State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong

E

Edward C. Holmes

T

Tommy T. Lam

State Key Laboratory of Emerging Infectious Diseases, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong