Pathogens spread by high-flying wind-borne mosquitoes

R Roland Bamou (Division of Intramural Research, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health) A Adama Dao (Malaria Research and Training Center/Faculty of Medicine, Pharmacy and Odonto-Stomatology University of Sciences, Techniques and Technologies of Bamako) A Alpha S. Yaro (Malaria Research and Training Center/Faculty of Medicine, Pharmacy and Odonto-Stomatology University of Sciences, Techniques and Technologies of Bamako) C Cedric Kouam (Division of Intramural Research, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health) K Koray Ergunay (Department of Entomology, Smithsonian Institution, National Museum of Natural History) B Brian P. Bourke (Department of Entomology, Smithsonian Institution, National Museum of Natural History) M Moussa Diallo (Malaria Research and Training Center/Faculty of Medicine, Pharmacy and Odonto-Stomatology University of Sciences, Techniques and Technologies of Bamako) Z Zana L. Sanogo (Malaria Research and Training Center/Faculty of Medicine, Pharmacy and Odonto-Stomatology University of Sciences, Techniques and Technologies of Bamako) S Samake Djibril (Malaria Research and Training Center/Faculty of Medicine, Pharmacy and Odonto-Stomatology University of Sciences, Techniques and Technologies of Bamako) Y Yaw A. Afrane (Department of Medical Microbiology, University of Ghana Medical School, University of Ghana) A Abdul Rahim Mohammed (Department of Medical Microbiology, University of Ghana Medical School, University of Ghana) C Christopher M. Owusu-Asenso (Department of Medical Microbiology, University of Ghana Medical School, University of Ghana) G Gabriel Akosah-Brempong (Department of Medical Microbiology, University of Ghana Medical School, University of Ghana) C Cosmos M. Pambit-Zong (Department of Medical Microbiology, University of Ghana Medical School, University of Ghana) B Benjamin J. Krajacich (Division of Intramural Research, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health) R Roy Faiman (Division of Intramural Research, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health) M M. Andreina Pacheco (Biology Department/Institute of Genomics and Evolutionary Medicine, Temple University) A Ananias A. Escalante (Biology Department/Institute of Genomics and Evolutionary Medicine, Temple University) S Scott C. Weaver (Department of Microbiology and Immunology, University of Texas Medical Branch) R Rita Nartey (Division of Intramural Research, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health) J Jason W. Chapman (Department of Entomology, College of Plant Protection, Nanjing Agricultural University) D Don R. Reynolds (Natural Resources Institute, University of Greenwich) Y Yvonne-Marie Linton (Department of Entomology, Smithsonian Institution, National Museum of Natural History) T Tovi Lehmann (Division of Intramural Research, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health)

Abstract

Mosquito-borne diseases such as malaria and dengue threaten billions of people and cause the death of hundreds of thousands annually. Recent studies have revealed that many mosquito species regularly engage in high-altitude wind-borne migration, but its epidemiological significance remains unclear. The hypothesis that high-flying mosquitoes spread pathogens over long distances has not been directly tested. Here, we report that high-flying mosquitoes are commonly infected with arboviruses, protozoans, and helminths and provide a insights into this pathogen–vector aerial network. A total of 1,017 female mosquitoes intercepted on nets suspended from helium balloons at 120 to 290 m above ground over Mali and Ghana were screened for infection with arboviruses, Haemosporida, and filariae. The mosquitoes collected at altitude comprised 61 species, across 10 genera, dominated by Culex , Aedes, and Anopheles . Infection and infectiousness (capacity to transmit a pathogen to another host inferred based on disseminated infection) rates of migrant mosquitoes were 7.2% and 4.4% with Plasmodium spp., 1.6% and 0.6% with filariae, and 3.5% and 1.1% with flaviviruses, respectively. Twenty-one mosquito-borne pathogens were identified, including Dengue, West Nile, and M’Poko viruses, 15 avian Plasmodium species including Plasmodium matutinum , and three filariids, including Pelecitus sp. Confirmed head–thorax (disseminated) infections of multiple pathogens in Culex perexiguus , Mansonia uniformis , and Anopheles squamosus reveal that pathogens carried by high-altitude wind-borne mosquitoes are capable of infecting hosts far from their departure location. This high-altitude traffic of sylvatic pathogens (circulating in wild animals) may be key to their maintenance among enzootic foci as well as initiating outbreaks at distant locations.

Article Details

Volume / Issue Vol. 122, Issue 48
Published December 02, 2025
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (24)

R

Roland Bamou

Division of Intramural Research, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health

A

Adama Dao

Malaria Research and Training Center/Faculty of Medicine, Pharmacy and Odonto-Stomatology University of Sciences, Techniques and Technologies of Bamako

A

Alpha S. Yaro

Malaria Research and Training Center/Faculty of Medicine, Pharmacy and Odonto-Stomatology University of Sciences, Techniques and Technologies of Bamako

C

Cedric Kouam

Division of Intramural Research, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health

K

Koray Ergunay

Department of Entomology, Smithsonian Institution, National Museum of Natural History

B

Brian P. Bourke

Department of Entomology, Smithsonian Institution, National Museum of Natural History

M

Moussa Diallo

Malaria Research and Training Center/Faculty of Medicine, Pharmacy and Odonto-Stomatology University of Sciences, Techniques and Technologies of Bamako

Z

Zana L. Sanogo

Malaria Research and Training Center/Faculty of Medicine, Pharmacy and Odonto-Stomatology University of Sciences, Techniques and Technologies of Bamako

S

Samake Djibril

Malaria Research and Training Center/Faculty of Medicine, Pharmacy and Odonto-Stomatology University of Sciences, Techniques and Technologies of Bamako

Y

Yaw A. Afrane

Department of Medical Microbiology, University of Ghana Medical School, University of Ghana

A

Abdul Rahim Mohammed

Department of Medical Microbiology, University of Ghana Medical School, University of Ghana

C

Christopher M. Owusu-Asenso

Department of Medical Microbiology, University of Ghana Medical School, University of Ghana

G

Gabriel Akosah-Brempong

Department of Medical Microbiology, University of Ghana Medical School, University of Ghana

C

Cosmos M. Pambit-Zong

Department of Medical Microbiology, University of Ghana Medical School, University of Ghana

B

Benjamin J. Krajacich

Division of Intramural Research, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health

R

Roy Faiman

Division of Intramural Research, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health

M

M. Andreina Pacheco

Biology Department/Institute of Genomics and Evolutionary Medicine, Temple University

A

Ananias A. Escalante

Biology Department/Institute of Genomics and Evolutionary Medicine, Temple University

S

Scott C. Weaver

Department of Microbiology and Immunology, University of Texas Medical Branch

R

Rita Nartey

Division of Intramural Research, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health

J

Jason W. Chapman

Department of Entomology, College of Plant Protection, Nanjing Agricultural University

D

Don R. Reynolds

Natural Resources Institute, University of Greenwich

Y

Yvonne-Marie Linton

Department of Entomology, Smithsonian Institution, National Museum of Natural History

T

Tovi Lehmann

Division of Intramural Research, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health