p63 and ZNF148 cooperate to regulate head and neck squamous cell carcinoma

R Rosalba Pecorari (Department of Experimental Medicine, Tor Vergata Oncoscience Research, University of Rome Tor Vergata) M Mara Mancini (Department of Experimental Medicine, University of Rome “Tor Vergata”) A Artem Smirnov (Department of Experimental Medicine, University of Rome “Tor Vergata”) G Giacomo Corleone (Struttura di Area Funzionale di Ricerca Unit (SAFU), Department of Research, Advanced Diagnostics, and Technological Innovation, Translational Research Area, Istituto di Ricovero e Cura a Carattere Scientifico Regina Elena National Cancer Institute) F Flavia Novelli (Department of Experimental Medicine, University of Rome “Tor Vergata”) A Anna Maria Lena (Department of Experimental Medicine, University of Rome “Tor Vergata”) M Mariacristina Franzese Canonico (Department of Experimental Medicine, University of Rome “Tor Vergata”) M Manuela Montanaro (Department of Experimental Medicine, University of Rome “Tor Vergata”) A Angela Cappello (Department of Experimental Medicine, University of Rome “Tor Vergata”) A Andrea Sacconi (Biostatistics, Bioinformatics and Clinical Trial Center, Istituto di Ricovero e Cura a Carattere Scientifico Regina Elena National Cancer Institute) G Gabriella Misso (Department of Precision Medicine, University of Campania Luigi Vanvitelli) M Michela Falco (Department of Precision Medicine, University of Campania Luigi Vanvitelli) C Chiara Tammaro F Fabiola Ciccosanti (National Institute for Infectious Diseases, Istituto di Ricovero e Cura a Carattere Scientifico “Lazzaro Spallanzani”) M Mauro Piacentini (National Institute for Infectious Diseases, Istituto di Ricovero e Cura a Carattere Scientifico “Lazzaro Spallanzani”) M Manuel Scimeca (Department of Experimental Medicine, University of Rome “Tor Vergata”) M Michele Caraglia (Department of Precision Medicine, University of Campania Luigi Vanvitelli) G Giovanni Blandino (Translational Oncology Research Unit, Department of Research, Diagnosis and Innovative Technologies, Istituto di Ricovero e Cura a Carattere Scientifico Regina Elena National Cancer Institute) A Alessandro Mauriello (Department of Experimental Medicine, University of Rome “Tor Vergata”) M Maurizio Fanciulli (Struttura di Area Funzionale di Ricerca Unit (SAFU), Department of Research, Advanced Diagnostics, and Technological Innovation, Translational Research Area, Istituto di Ricovero e Cura a Carattere Scientifico Regina Elena National Cancer Institute) G Gerry Melino (Department of Experimental Medicine, TOR, University of Rome “Tor Vergata”) E Eleonora Candi (Department of Experimental Medicine, University of Rome “Tor Vergata”)

Abstract

Head and neck squamous cell carcinoma (HNSCC) is a common and aggressive malignancy. While significant advances have been made in the management of low-grade cancer, treatment of advanced HNSCC remains challenging. Here, we used a proteomic approach to find binding partners of the oncogene p63, the most frequently amplified transcription factor in HNSCC. We identified a zinc finger protein, ZNF148, which is coexpressed and physically binds p63 in carcinoma cells. Genome occupancy analyses identified a functional transcribed enhancer-derived RNA (eRNA) upstream of the CCND1 gene occupied by both factors. Mechanistically, p63 and ZNF148 control transcription of this eRNA, leading to overexpression of cyclin D1 to reinforce tumor cell proliferation. Importantly, this axis is specific for cancer cells and remains inactive in normal epithelial cells. The expression levels of these factors and the eRNA are positively correlated in cancer and associated with advanced stage and metastasis. Collectively, our data reveal a molecular pathway controlling HNSCC progression and identify potential selective targets for cancer treatment.

Article Details

Volume / Issue Vol. 122, Issue 28
Published July 15, 2025
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (22)

R

Rosalba Pecorari

Department of Experimental Medicine, Tor Vergata Oncoscience Research, University of Rome Tor Vergata

M

Mara Mancini

Department of Experimental Medicine, University of Rome “Tor Vergata”

A

Artem Smirnov

Department of Experimental Medicine, University of Rome “Tor Vergata”

G

Giacomo Corleone

Struttura di Area Funzionale di Ricerca Unit (SAFU), Department of Research, Advanced Diagnostics, and Technological Innovation, Translational Research Area, Istituto di Ricovero e Cura a Carattere Scientifico Regina Elena National Cancer Institute

F

Flavia Novelli

Department of Experimental Medicine, University of Rome “Tor Vergata”

A

Anna Maria Lena

Department of Experimental Medicine, University of Rome “Tor Vergata”

M

Mariacristina Franzese Canonico

Department of Experimental Medicine, University of Rome “Tor Vergata”

M

Manuela Montanaro

Department of Experimental Medicine, University of Rome “Tor Vergata”

A

Angela Cappello

Department of Experimental Medicine, University of Rome “Tor Vergata”

A

Andrea Sacconi

Biostatistics, Bioinformatics and Clinical Trial Center, Istituto di Ricovero e Cura a Carattere Scientifico Regina Elena National Cancer Institute

G

Gabriella Misso

Department of Precision Medicine, University of Campania Luigi Vanvitelli

M

Michela Falco

Department of Precision Medicine, University of Campania Luigi Vanvitelli

C

Chiara Tammaro

F

Fabiola Ciccosanti

National Institute for Infectious Diseases, Istituto di Ricovero e Cura a Carattere Scientifico “Lazzaro Spallanzani”

M

Mauro Piacentini

National Institute for Infectious Diseases, Istituto di Ricovero e Cura a Carattere Scientifico “Lazzaro Spallanzani”

M

Manuel Scimeca

Department of Experimental Medicine, University of Rome “Tor Vergata”

M

Michele Caraglia

Department of Precision Medicine, University of Campania Luigi Vanvitelli

G

Giovanni Blandino

Translational Oncology Research Unit, Department of Research, Diagnosis and Innovative Technologies, Istituto di Ricovero e Cura a Carattere Scientifico Regina Elena National Cancer Institute

A

Alessandro Mauriello

Department of Experimental Medicine, University of Rome “Tor Vergata”

M

Maurizio Fanciulli

Struttura di Area Funzionale di Ricerca Unit (SAFU), Department of Research, Advanced Diagnostics, and Technological Innovation, Translational Research Area, Istituto di Ricovero e Cura a Carattere Scientifico Regina Elena National Cancer Institute

G

Gerry Melino

Department of Experimental Medicine, TOR, University of Rome “Tor Vergata”

E

Eleonora Candi

Department of Experimental Medicine, University of Rome “Tor Vergata”