Overexpression of hsa-HLA-DRB1 may delay diabetic wound healing and angiogenesis by regulating miRNA_12118 and FLT-1

Abstract

Abstract We investigated the role and mechanism of hsa-HLA-DRB1 in the development and progression of diabetic foot ulcers. High-throughput sequencing was performed on three normal foot trauma tissues and diabetic foot ulcer tissues. The circRNAs with significant differences were identified. The downstream miRNAs were predicted by miRanda and RNAhybrid databases, and the mRNAs were predicted by the TargetScan database. Validation was performed with CCK8, flow cytometry, trabecular scratch assay, tubule generation assay, Western blot, dual luciferase assay, and RT-qPCR. High-throughput sequencing identified 461 significantly different circRNAs, of which 260 were up-regulated and 201 down-regulated. Compared to normal tissue, hsa-HLA-DRB1 was highly expressed in diabetic foot ulcers. The hsa-HLA-DRB1/miRNA_12118/FLT-1 axis was constructed. In vitro, we found that HLA-DRB1 overexpression inhibited cell viability, wound healing, and tubule formation, promoted apoptosis, and enhanced FLT-1 expression in HUVECs. The upregulation of hsa-HLA-DRB1 may promote diabetic foot development The upregulation of hsa - HLA - DRB1 may inhibit the biological function of endothelial cells by targeting miRNA_12118 and acting on FLT − 1. by targeting miRNA_12118 and acting on FLT-1. Therefore, our study highlights the key role of the hsa-HLA-DRB1/miRNA_12118/FLT-1 axis in diabetic foot trauma.