Outcomes of BCP-ALL with hypodiploidy or <i>BCR</i> :: <i>ABL1</i> fusion in children undergoing allogeneic HSCT: results from the FORUM study

J Jochen Buechner (1Department of Pediatric Hematology and Oncology, Oslo University Hospital, Oslo, Norway) U Ulrike Poetschger (2St. Anna Children's Cancer Research Institute, Vienna, Austria) P Peter Bader (3Division for Stem Cell Transplantation, Immunology, Department of Pediatrics, Goethe University, University Hospital, Frankfurt am Main, Germany) A Akif Yeşilipek (4Pediatric Stem Cell Transplantation Unit, Antalya Medical Park Hospital, Antalya, Turkey) H Herbert Pichler (2St. Anna Children's Cancer Research Institute, Vienna, Austria) J Julia Palma (6Department of Pediatrics–Bone Marrow Transplantation Unit, Faculty of Medicine, Hospital Luis Calvo Mackenna, University of Chile, Santiago, Chile) R Raquel Staciuk (7Hospital de Pediatría Prof. Dr. Juan P. Garrahan, Buenos Aires, Argentina) P Petr Riha (9Department of Pediatric Hematology and Oncology, Motol University Hospital, Prague, Czech Republic) G Gergely Kriván (10Pediatric Hematology and Stem Cell Transplantation Department, Central Hospital of Southern Pest, National Institute of Hematology and Infectious Diseases, Budapest, Hungary) M Marianne Ifversen (11Department of Pediatric and Adolescent Medicine, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark) T Tayfun Güngör (12Department of Hematology/Oncology/Immunology, Gene Therapy, and Stem Cell Transplantation, Eleonore Foundation and Children’s Research Center, University Children’s Hospital, Zürich, Switzerland) E Evgenios Goussetis (13Stem Cell Transplant Unit, Agia Sofia Children's Hospital, Athens, Greece) K Krzysztof Kalwak (14Department of Pediatric Bone Marrow Transplantation, Oncology, and Hematology, Wroclaw Medical University, Wroclaw, Poland) J Jacek Toporski (15Department Cell Therapy and Allogeneic Stem Cell Transplant, Karolinska University Hospital, Stockholm, Sweden) M Melissa Gabriel (16The Children's Hospital at Westmead, Sydney, Australia) M Marleen Renard (17University Hospital Leuven, Leuven, Belgium) C Cristina Diaz-de-Heredia (18Department of Pediatric Hematology and Oncology, Hospital Universitari Vall d’Hebron, Vall d’Hebron Institut de Recerca, Barcelona, Spain) T Toni Matic (19Department of Pediatrics, University Hospital Center Zagreb, Zagreb, Croatia) F Friso G. Calkoen P Peter Švec (Institute of Physics, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 11 Bratislava, Slovakia) R Roland Meisel (Division of Pediatric Stem Cell Therapy, Department of Pediatric Oncology, Hematology and Clinical Immunology, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany) A Adriana Balduzzi (23Pediatric Hematopoietic Transplant Unit, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy) F Franco Locatelli (IRCCS Ospedale Pediatrico Bambino Gesù Rome, Rome) C Christina Peters (2St. Anna Children's Cancer Research Institute, Vienna, Austria) J Jean-Hugues Dalle (27Hemato-Immunology Department, Robert Debré Academic Hospital, GHU AP-HP Nord Université Paris Cité, Paris, France) J Jerry Stein (28Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel)

Abstract

Abstract Hypodiploid and BCR::ABL1+ B-cell precursor acute lymphoblastic leukemia (BCP-ALL) confers a high risk of disease relapse. We investigated post–hematopoietic stem cell transplantation (HSCT) outcomes within the prospective FORUM trial, comparing outcomes among these genetic subgroups with those of patients without these lesions. The use of pre- and post-HSCT add-on treatments, including tyrosine kinase inhibitors (TKI) and immunotherapies, was also assessed. Multivariate analysis evaluated associations with overall survival (OS), event-free survival (EFS), and cumulative incidence of relapse (CIR). The FORUM trial enrolled 741 patients aged ≥4 years with BCP-ALL who underwent HSCT from HLA-matched donors (2013-2023). The 3-year OS and EFS did not differ significantly between patients with BCR::ABL1 fusion, hypodiploidy, and neither of these 2 genetic lesions. However, patients with hypodiploid BCP-ALL in second complete remission (CR2) showed inferior OS and EFS, driven by higher nonrelapse mortality (NRM), which occurred exclusively in near-diploid cases. No NRM occurred in severe hypodiploid cases conditioned with total body irradiation. Minimal residual disease (MRD) positivity at transplant predicted worse OS, EFS, and CIR in all genetic groups. Patients with hypodiploid BCP-ALL were difficult to salvage after relapse, even with chimeric antigen receptor T-cell therapy. By contrast, BCR::ABL1+ patients had favorable outcomes, even when MRD positive before HSCT. Prophylactic TKI use after HSCT improved EFS and reduced CIR. BCR::ABL1+ patients who received a transplant in CR2 had a 3-year OS of 96%. In conclusion, the standardized FORUM protocol yielded comparable outcomes across genetic subgroups. Posttransplant TKI maintenance improved outcomes in BCR::ABL1+ BCP-ALL. This trial was registered at www.clinicaltrials.gov as #NCT01949129 and at www.clinicaltrialsregister.eu as #EudraCT2012-0032-22.

Article Details

Journal Blood
Volume / Issue Vol. 147, Issue 12
Published March 19, 2026
Pages 1365-1379
ISSN 0006-4971
Publisher Elsevier BV

Journal Info

Blood

Elsevier BV

ISSN: 0006-4971 Health Sciences

Authors (26)

J

Jochen Buechner

1Department of Pediatric Hematology and Oncology, Oslo University Hospital, Oslo, Norway

U

Ulrike Poetschger

2St. Anna Children's Cancer Research Institute, Vienna, Austria

P

Peter Bader

3Division for Stem Cell Transplantation, Immunology, Department of Pediatrics, Goethe University, University Hospital, Frankfurt am Main, Germany

A

Akif Yeşilipek

4Pediatric Stem Cell Transplantation Unit, Antalya Medical Park Hospital, Antalya, Turkey

H

Herbert Pichler

2St. Anna Children's Cancer Research Institute, Vienna, Austria

J

Julia Palma

6Department of Pediatrics–Bone Marrow Transplantation Unit, Faculty of Medicine, Hospital Luis Calvo Mackenna, University of Chile, Santiago, Chile

R

Raquel Staciuk

7Hospital de Pediatría Prof. Dr. Juan P. Garrahan, Buenos Aires, Argentina

P

Petr Riha

9Department of Pediatric Hematology and Oncology, Motol University Hospital, Prague, Czech Republic

G

Gergely Kriván

10Pediatric Hematology and Stem Cell Transplantation Department, Central Hospital of Southern Pest, National Institute of Hematology and Infectious Diseases, Budapest, Hungary

M

Marianne Ifversen

11Department of Pediatric and Adolescent Medicine, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark

T

Tayfun Güngör

12Department of Hematology/Oncology/Immunology, Gene Therapy, and Stem Cell Transplantation, Eleonore Foundation and Children’s Research Center, University Children’s Hospital, Zürich, Switzerland

E

Evgenios Goussetis

13Stem Cell Transplant Unit, Agia Sofia Children's Hospital, Athens, Greece

K

Krzysztof Kalwak

14Department of Pediatric Bone Marrow Transplantation, Oncology, and Hematology, Wroclaw Medical University, Wroclaw, Poland

J

Jacek Toporski

15Department Cell Therapy and Allogeneic Stem Cell Transplant, Karolinska University Hospital, Stockholm, Sweden

M

Melissa Gabriel

16The Children's Hospital at Westmead, Sydney, Australia

M

Marleen Renard

17University Hospital Leuven, Leuven, Belgium

C

Cristina Diaz-de-Heredia

18Department of Pediatric Hematology and Oncology, Hospital Universitari Vall d’Hebron, Vall d’Hebron Institut de Recerca, Barcelona, Spain

T

Toni Matic

19Department of Pediatrics, University Hospital Center Zagreb, Zagreb, Croatia

F

Friso G. Calkoen

P

Peter Švec

Institute of Physics, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 11 Bratislava, Slovakia

R

Roland Meisel

Division of Pediatric Stem Cell Therapy, Department of Pediatric Oncology, Hematology and Clinical Immunology, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany

A

Adriana Balduzzi

23Pediatric Hematopoietic Transplant Unit, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy

F

Franco Locatelli

IRCCS Ospedale Pediatrico Bambino Gesù Rome, Rome

C

Christina Peters

2St. Anna Children's Cancer Research Institute, Vienna, Austria

J

Jean-Hugues Dalle

27Hemato-Immunology Department, Robert Debré Academic Hospital, GHU AP-HP Nord Université Paris Cité, Paris, France

J

Jerry Stein

28Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel