OsNLP3 and OsPHR2 orchestrate direct and mycorrhizal pathways for nitrate uptake by regulating NAR2.1–NRT2s complexes in rice
Abstract
Nitrogen (N) is the most important essential nutrient required by plants. Most land plants have evolved two N uptake pathways, a direct root pathway and a symbiotic pathway, via association with arbuscular mycorrhizal (AM) fungi. However, the interaction between the two pathways is ambiguous. Here, we report that OsNAR2.1–OsNRT2s, the nitrate (NO 3 − ) transporter complexes with crucial roles in direct NO 3 − uptake, are also recruited for symbiotic NO 3 − uptake. OsNAR2.1 and OsNRT2.1/2.2 are coregulated by NIN-like protein 3 (OsNLP3), a key regulator in NO 3 − signaling, and OsPHR2, a major regulator of phosphate starvation responses. More importantly, AM symbiosis induces expression of OsNAR2.1 – OsNRT2s, OsNLP3, and OsSPX4 , encoding an intracellular Pi sensor, in arbuscular-containing cells, but weakens their expression in the epidermis. OsNAR2.1 and OsNLP3 can activate both mycorrhizal NO 3 − uptake and mycorrhization efficiency. Overall, we demonstrate that OsNLP3 and OsPHR2 orchestrate the direct and mycorrhizal NO 3 − uptake pathways by regulating the NAR2.1–NRT2s complexes in rice.
Article Details
Journal Info
Proceedings of the National Academy of Sciences
National Academy of Sciences
Authors (14)
Shuangshuang Wang
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Department of Plant Nutrition, College of Resources and Environmental Sciences, Nanjing Agricultural University
Hanghang Ye
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Department of Plant Nutrition, College of Resources and Environmental Sciences, Nanjing Agricultural University
Congfan Yang
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Department of Plant Nutrition, College of Resources and Environmental Sciences, Nanjing Agricultural University
Yan Zhang
Jiawen Pu
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Department of Plant Nutrition, College of Resources and Environmental Sciences, Nanjing Agricultural University
Yuhan Ren
Kun Xie
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Department of Plant Nutrition, College of Resources and Environmental Sciences, Nanjing Agricultural University
Lingxiao Wang
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Department of Plant Nutrition, College of Resources and Environmental Sciences, Nanjing Agricultural University
Dechao Zeng
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Department of Plant Nutrition, College of Resources and Environmental Sciences, Nanjing Agricultural University
Haoqiang He
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Department of Plant Nutrition, College of Resources and Environmental Sciences, Nanjing Agricultural University
Haoyan Ji
Luis Rafael Herrera-Estrella
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Department of Plant Nutrition, College of Resources and Environmental Sciences, Nanjing Agricultural University
Guohua Xu
State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging, National Center for Magnetic Resonance in Wuhan, Wuhan National Laboratory for Optoelectronics, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology
Aiqun Chen
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, Department of Plant Nutrition, College of Resources and Environmental Sciences, Nanjing Agricultural University