Optimal therapy for pediatric B-ALL in first relapse: Comparative effectiveness of blinatumomab to hematopoietic cell transplantation versus CD19 CAR T cell therapy

R Regina Myers (1Children's Hospital of Philadelphia, Division of Oncology, Philadelphia, United States) Y Yimei Li H Hongyan Liu (CAS Key Laboratory of Green Process and Engineering, State Key Laboratory of Multiphase Complex Systems, Beijing Key Laboratory of Ionic Liquids Clean Process, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China) A Alex Hoover L Laura Shinehouse (1Children's Hospital of Philadelphia, Division of Oncology, Philadelphia, United States) A Ashley Hinson (3Atrium Health Levine Children's Hospital, Department of Pediatric Hematology and Oncology, Charlotte, United States) M Mira Kohorst (4Mayo Clinic Children's, Division of Pediatric Hematology-Oncology, Rochester, United States) A Anna Elias (4Mayo Clinic Children's, Division of Pediatric Hematology-Oncology, Rochester, United States) E Ella Keenan (1Children's Hospital of Philadelphia, Division of Oncology, Philadelphia, United States) H Hannah Acres (5University of Texas Southwestern Medical Center, Department of Pediatrics, Dallas, United States) I Ibrahim Ahmed A Abdulla Al-Mulla (7Children's Hospital of Richmond at VCU, Division of Oncology, Richmond, United States) L Laura Arthur (8Lurie Children's Hospital, Division of Hematology, Oncology, Neuro-Oncology, & Stem-Cell Transplant, Chicago, United States) J Jill Beck (9University of Nebraska Medical Center, Division of Pediatric Hematology/Oncology, Omaha, United States) A Abigale Berry (1Children's Hospital of Philadelphia, Division of Oncology, Philadelphia, United States) D Deepika Bhatla (10Cardinal Glennon Children's Hospital, Division of Oncology, Saint Louis, United States) E Eliza Briscoe (11University of Utah/Primary Children's Hospital, Department of Pediatrics, Salt Lake City, United States) G Grace Chang R Roland Chu (13Children's Hospital of Michigan, Division of Pediatric Hematology/Oncology, Detroit, United States) L Laurie Davis (14Baylor College of Medicine, Division of Blood and Marrow Transplant, San Antonio, United States) J J. Gregory Dolan (22Division of Hematology and Oncology, Intermountain Primary Children's Hospital, Huntsman Cancer Institute, Spencer Fox Eccles School of Medicine, University of Utah, Salt Lake City, United States) L Lyannette Elo (14Baylor College of Medicine, Division of Blood and Marrow Transplant, San Antonio, United States) E Elizabeth Eom (15Children's Hospital Los Angeles, Division of Hematology-Oncology, Los Angeles, United States) A Alejandra Escobar Vasco (16Medical College of Wisconsin, Division of Pediatric Hematology/Oncology/Blood and Marrow Transplant, Department of Pediatrics, Milwaukee, United States) V Vanessa Fabrizio (6Division of Pediatric Hematology-Oncology-BMT, University of Colorado, Aurora, United States) K Kelly Faulk (17Children's Hospital of Colorado, Center for Cancer and Blood Disorders, Department of Pediatrics, Aurora, United States) V Victor Galan-Gomez (18Hospital Universitario La Paz, Pediatric Hemato-Oncology Unit, Madrid, Spain) E Erin Goode (2Peter MacCallum Cancer Centre, Department of Pathology, Melbourne, Australia) S Stephan Grupp (1Children's Hospital of Philadelphia, Division of Oncology, Philadelphia, United States) A Anurekha Hall (12Seattle Children's Hospital, Seattle, WA, Division of Oncology, Seattle, United States) D Darcy Hamill S Smitha Vasanna (20Arkansas Children's Hospital, Pediatric Oncology, Bone Marrow Transplant and Cellular Therapies, Little Rock, United States) H Hannah Kinoshita (21Children's National Hospital, Center for Cancer and Blood Disorders, Washington, United States) E Elizabeth Krieger (3Virginia Commonwealth University, Department of Pediatrics, Richmond, United States) C Cathy Lee-Miller (22University of Wisconsin School of Medicine and Public Health, Department of Pediatrics, Division of Hematology, Oncology, Transplant & Cell Therapy, Madison, United States) K Katherine Lind (17Children's Hospital of Colorado, Center for Cancer and Blood Disorders, Department of Pediatrics, Aurora, United States) J Jordan Milner (11Department of Pediatrics, Division of Hematology/Oncology, University of Florida, UF Health Shands Children's Hospital, Gainesville, United States) G Giselle Moore-Higgs (23University of Florida, Division of Oncology, Gainesville, United States) A Amy Moskop (16Medical College of Wisconsin, Division of Pediatric Hematology/Oncology/Blood and Marrow Transplant, Department of Pediatrics, Milwaukee, United States) L Lindsey Murphy (1City of Hope, Pediatrics, Duarte, United States) M Megan Murphy (25Johns Hopkins Hospital, Division of Pediatric Oncology, Department of Oncology, Baltimore, United States) G Gabriella Nguyen (5University of Texas Southwestern Medical Center, Department of Pediatrics, Dallas, United States) M Maria Ortega (19Nemours Children's Health, Lisa Dean Moseley Foundation for Cancer and Blood Disorders, Wilmington, United States) H Hiren Patel (26Cohen Children's Medical Center, Division of Pediatric Hematology/Oncology and Cellular Therapy, New Hyde Park, United States) A Antonio Perez (18Hospital Universitario La Paz, Pediatric Hemato-Oncology Unit, Madrid, Spain) T Thomas Pfeiffer A Alexandra Prosser-Dombrowski (6Children's Mercy Kansas City, Division of Hematology/Oncology/Bone Marrow Transplant, Kansas City, United States) M Mahvish Rahim (28Children's Hospital at Montefiore, Division of Pediatric Hematology, Oncology, and Stem Cell Transplant, Bronx, United States) A April Rahrig (29Riley Hospital for Children, Division of Pediatric Hematology, Oncology and Stem Cell Transplant, Indianapolis, United States) S Shanti Ramachandran (30Perth Children's Hospital, Department of Clinical Haematology, Oncology and Bone Marrow Transplantation, Nedlands, Australia) M Miza Salim Hammoud A Amanda Saraf (1Riley Children's Health, Indianapolis, United States) J Jamie Shoag (31Cleveland Clinic Children's, Division of Pediatric Hematology and Oncology, Cleveland, United States) H Heather Symons (25Johns Hopkins Hospital, Division of Pediatric Oncology, Department of Oncology, Baltimore, United States) N Nicholas Tastet (3Atrium Health Levine Children's Hospital, Department of Pediatric Hematology and Oncology, Charlotte, United States) S Stephanie Thomas (20Arkansas Children's Hospital, Pediatric Oncology, Bone Marrow Transplant and Cellular Therapies, Little Rock, United States) M Molly Thornock (2City of Hope, Population Sciences, Duarte, United States) K Keri Toner (21Children's National Hospital, Center for Cancer and Blood Disorders, Washington, United States) A Allison Weisnicht (22University of Wisconsin School of Medicine and Public Health, Department of Pediatrics, Division of Hematology, Oncology, Transplant & Cell Therapy, Madison, United States) S Sara Zarnegar-Lumley (8Lurie Children's Hospital, Division of Hematology, Oncology, Neuro-Oncology, & Stem-Cell Transplant, Chicago, United States) N Nirali Shah (32National Cancer Institute, Pediatric Oncology Branch, Bethesda, United States) S Shannon Maude (1Children's Hospital of Philadelphia, Division of Oncology, Philadelphia, United States) J Jeremy Rubinstein (33Cincinnati Children's Hospital Medical Center, Division of Oncology, Cincinnati, United States) T Troy Quigg (2Section of Pediatric Bone Marrow Transplantation and Cellular Therapy, Helen DeVos Children's Hospital, Grand Rapids, United States) D Deepa Bhojwani C Caitlin Elgarten (University of Pennsylvania, Philadelphia)

Abstract

Abstract INTRODUCTION Outcomes for 1st relapse of pediatric B-lymphoblastic leukemia (B-ALL), especially high/intermediate-risk (HR/IR) relapse, remain unacceptably poor. Limited enrollment of this population on cooperative group clinical trials has impeded our ability to study potentially effective regimens. In the current era, patients can access innovative immunotherapies commercially. However, there is no mechanism to capture outcomes for these off-study patients and no comparative effectiveness data to guide treatment selection. METHODS We conducted a multicenter, retrospective study of patients <30 years who initiated 1st relapse therapy at a participating center from 2018-2022 (ReCALL-1 Study). Objectives were to define real-world survival outcomes, compare response rates of reinduction strategies, describe the post-reinduction treatment landscape, and, for patients with HR/IR relapse, assess the comparative effectiveness (CE) of blinatumomab to hematopoietic cell transplant (blina/HCT) v CD19 CAR T cells (CART). For the CE analysis, we emulated an open-label, randomized study of blina/HCT v CART who achieved an M1/M2 marrow at the end of an intensive reinduction (EoRI). Patients were classified by their intended treatment at EoRI. Cox regression models were used to adjust for unbalanced patient and disease features by arms. The primary outcome was overall survival (OS) from EoRI. RESULTS The full cohort included 479 patients from 33 centers (31 US, 2 international). Median time to relapse was 31 months (m); 77% had bone marrow (BM) involvement and 31% central nervous sytem involvement. With a median follow-up of 49m from relapse, 4 year (y) OS, event-free survival (EFS), and non-relapse mortality (NRM) were 64%, 43%, and 13%, respectively. Among patients with very early BM (<18m, n=79), early BM (18-35m, n=76), late BM (≥36m, n=162), and isolated CNS (n=85) relapses, 4y OS/EFS were 29%/20%, 57%/35%, 78%/49%, and 84%/61%, respectively. Only 19% received reinduction therapy on a clinical trial. Among patients with BM disease, R3 reinduction (n=179) and VXLD (n=61) had identical MRD-negative (<0.01%) CR rates of 38% (p=1.00) and equivalent treatment failure (TF; M3 or extramedullary disease) rates: R3, 8% v VXLD 7%, p=0.79. Blina (n=33) and inotuzumab-based (n=21) reinductions achieved MRD-negative CR rates of 39% and 52%, respectively, but higher TF rates of 39% and 24%. Reinduction mortality was 2%: R3, n=5; VXLD, n=3; 3-drug reinduction, n=1. Overall, as part of 1st relapse therapy, 36% received CART (78% commercial product), 44% HCT, 40% blina, and 11% inotuzumab. The emulated trial included 159 patients, 95 on blina/HCT and 64 on CART. Study arms were well matched by demographics, cytogenetics, and relapse sites, but CART patients were more likely to have relapsed <18m from diagnosis (34% v 24%) and have EoRI M2 marrows (31% v 11%), and less likely to have private insurance (36% v 51%). Unadjusted OS was comparable between arms (log rank p=0.18): CART, 60% at 4y; blina/HCT, 71%, as was NRM (10% v 13% at 4y). EFS was lower for CART (p=0.049): 4y EFS 35% v 49% for blina/HCT. After adjustment for unbalanced covariates, the hazard of death was equivalent for CART as compared to blina/HCT (aHR 1.1, 95% CI 0.6-2.1, p=0.67) and EFS became more comparable (aHR 1.3, 95% CI 0.8-2.1, p=0.22). 91% of blina/HCT and 97% of CART received their intended cell therapy; time from EoRI to cell therapy was shorter for CART (67 v 86 days, p<.001). In the CART arm, 34% underwent consolidative HCT; 1/3 were preplanned and 2/3 due to loss of B cell aplasia or NGS-MRD positivity. Of those with a 2nd relapse, CART patients were more likely to be CD19 negative (47% v 10%, p=0.003). CONCLUSION In this multicenter, real-world cohort of children treated for 1st relapse in the immunotherapy era, suboptimal outcomes for early BM relapse persisted, with particularly dismal 4y OS of 29% for relapses <18m after diagnosis. In contrast, survival for late BM and isolated CNS relapses compared favorably to historic data. Only 1 in 5 patients initiated relapse therapy on a clinical trial and although 1st relapse is not an approved indication for commercial CART in pediatrics, 36% received CART. In an emulated trial of post-reinduction blina/HCT v CART, adjusted EFS and OS were comparable, suggesting that CART may be a viable alternative to HCT for select patients. Post hoc analyses will identify whether specific subgroups may benefit more from one strategy.

Article Details

Journal Blood
Volume / Issue Vol. 146, Issue Supplement 1
Published November 03, 2025
Pages 3339-3339
ISSN 0006-4971
Publisher Elsevier BV

Journal Info

Blood

Elsevier BV

ISSN: 0006-4971 Health Sciences

Authors (66)

R

Regina Myers

1Children's Hospital of Philadelphia, Division of Oncology, Philadelphia, United States

Y

Yimei Li

H

Hongyan Liu

CAS Key Laboratory of Green Process and Engineering, State Key Laboratory of Multiphase Complex Systems, Beijing Key Laboratory of Ionic Liquids Clean Process, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

A

Alex Hoover

L

Laura Shinehouse

1Children's Hospital of Philadelphia, Division of Oncology, Philadelphia, United States

A

Ashley Hinson

3Atrium Health Levine Children's Hospital, Department of Pediatric Hematology and Oncology, Charlotte, United States

M

Mira Kohorst

4Mayo Clinic Children's, Division of Pediatric Hematology-Oncology, Rochester, United States

A

Anna Elias

4Mayo Clinic Children's, Division of Pediatric Hematology-Oncology, Rochester, United States

E

Ella Keenan

1Children's Hospital of Philadelphia, Division of Oncology, Philadelphia, United States

H

Hannah Acres

5University of Texas Southwestern Medical Center, Department of Pediatrics, Dallas, United States

I

Ibrahim Ahmed

A

Abdulla Al-Mulla

7Children's Hospital of Richmond at VCU, Division of Oncology, Richmond, United States

L

Laura Arthur

8Lurie Children's Hospital, Division of Hematology, Oncology, Neuro-Oncology, & Stem-Cell Transplant, Chicago, United States

J

Jill Beck

9University of Nebraska Medical Center, Division of Pediatric Hematology/Oncology, Omaha, United States

A

Abigale Berry

1Children's Hospital of Philadelphia, Division of Oncology, Philadelphia, United States

D

Deepika Bhatla

10Cardinal Glennon Children's Hospital, Division of Oncology, Saint Louis, United States

E

Eliza Briscoe

11University of Utah/Primary Children's Hospital, Department of Pediatrics, Salt Lake City, United States

G

Grace Chang

R

Roland Chu

13Children's Hospital of Michigan, Division of Pediatric Hematology/Oncology, Detroit, United States

L

Laurie Davis

14Baylor College of Medicine, Division of Blood and Marrow Transplant, San Antonio, United States

J

J. Gregory Dolan

22Division of Hematology and Oncology, Intermountain Primary Children's Hospital, Huntsman Cancer Institute, Spencer Fox Eccles School of Medicine, University of Utah, Salt Lake City, United States

L

Lyannette Elo

14Baylor College of Medicine, Division of Blood and Marrow Transplant, San Antonio, United States

E

Elizabeth Eom

15Children's Hospital Los Angeles, Division of Hematology-Oncology, Los Angeles, United States

A

Alejandra Escobar Vasco

16Medical College of Wisconsin, Division of Pediatric Hematology/Oncology/Blood and Marrow Transplant, Department of Pediatrics, Milwaukee, United States

V

Vanessa Fabrizio

6Division of Pediatric Hematology-Oncology-BMT, University of Colorado, Aurora, United States

K

Kelly Faulk

17Children's Hospital of Colorado, Center for Cancer and Blood Disorders, Department of Pediatrics, Aurora, United States

V

Victor Galan-Gomez

18Hospital Universitario La Paz, Pediatric Hemato-Oncology Unit, Madrid, Spain

E

Erin Goode

2Peter MacCallum Cancer Centre, Department of Pathology, Melbourne, Australia

S

Stephan Grupp

1Children's Hospital of Philadelphia, Division of Oncology, Philadelphia, United States

A

Anurekha Hall

12Seattle Children's Hospital, Seattle, WA, Division of Oncology, Seattle, United States

D

Darcy Hamill

S

Smitha Vasanna

20Arkansas Children's Hospital, Pediatric Oncology, Bone Marrow Transplant and Cellular Therapies, Little Rock, United States

H

Hannah Kinoshita

21Children's National Hospital, Center for Cancer and Blood Disorders, Washington, United States

E

Elizabeth Krieger

3Virginia Commonwealth University, Department of Pediatrics, Richmond, United States

C

Cathy Lee-Miller

22University of Wisconsin School of Medicine and Public Health, Department of Pediatrics, Division of Hematology, Oncology, Transplant & Cell Therapy, Madison, United States

K

Katherine Lind

17Children's Hospital of Colorado, Center for Cancer and Blood Disorders, Department of Pediatrics, Aurora, United States

J

Jordan Milner

11Department of Pediatrics, Division of Hematology/Oncology, University of Florida, UF Health Shands Children's Hospital, Gainesville, United States

G

Giselle Moore-Higgs

23University of Florida, Division of Oncology, Gainesville, United States

A

Amy Moskop

16Medical College of Wisconsin, Division of Pediatric Hematology/Oncology/Blood and Marrow Transplant, Department of Pediatrics, Milwaukee, United States

L

Lindsey Murphy

1City of Hope, Pediatrics, Duarte, United States

M

Megan Murphy

25Johns Hopkins Hospital, Division of Pediatric Oncology, Department of Oncology, Baltimore, United States

G

Gabriella Nguyen

5University of Texas Southwestern Medical Center, Department of Pediatrics, Dallas, United States

M

Maria Ortega

19Nemours Children's Health, Lisa Dean Moseley Foundation for Cancer and Blood Disorders, Wilmington, United States

H

Hiren Patel

26Cohen Children's Medical Center, Division of Pediatric Hematology/Oncology and Cellular Therapy, New Hyde Park, United States

A

Antonio Perez

18Hospital Universitario La Paz, Pediatric Hemato-Oncology Unit, Madrid, Spain

T

Thomas Pfeiffer

A

Alexandra Prosser-Dombrowski

6Children's Mercy Kansas City, Division of Hematology/Oncology/Bone Marrow Transplant, Kansas City, United States

M

Mahvish Rahim

28Children's Hospital at Montefiore, Division of Pediatric Hematology, Oncology, and Stem Cell Transplant, Bronx, United States

A

April Rahrig

29Riley Hospital for Children, Division of Pediatric Hematology, Oncology and Stem Cell Transplant, Indianapolis, United States

S

Shanti Ramachandran

30Perth Children's Hospital, Department of Clinical Haematology, Oncology and Bone Marrow Transplantation, Nedlands, Australia

M

Miza Salim Hammoud

A

Amanda Saraf

1Riley Children's Health, Indianapolis, United States

J

Jamie Shoag

31Cleveland Clinic Children's, Division of Pediatric Hematology and Oncology, Cleveland, United States

H

Heather Symons

25Johns Hopkins Hospital, Division of Pediatric Oncology, Department of Oncology, Baltimore, United States

N

Nicholas Tastet

3Atrium Health Levine Children's Hospital, Department of Pediatric Hematology and Oncology, Charlotte, United States

S

Stephanie Thomas

20Arkansas Children's Hospital, Pediatric Oncology, Bone Marrow Transplant and Cellular Therapies, Little Rock, United States

M

Molly Thornock

2City of Hope, Population Sciences, Duarte, United States

K

Keri Toner

21Children's National Hospital, Center for Cancer and Blood Disorders, Washington, United States

A

Allison Weisnicht

22University of Wisconsin School of Medicine and Public Health, Department of Pediatrics, Division of Hematology, Oncology, Transplant & Cell Therapy, Madison, United States

S

Sara Zarnegar-Lumley

8Lurie Children's Hospital, Division of Hematology, Oncology, Neuro-Oncology, & Stem-Cell Transplant, Chicago, United States

N

Nirali Shah

32National Cancer Institute, Pediatric Oncology Branch, Bethesda, United States

S

Shannon Maude

1Children's Hospital of Philadelphia, Division of Oncology, Philadelphia, United States

J

Jeremy Rubinstein

33Cincinnati Children's Hospital Medical Center, Division of Oncology, Cincinnati, United States

T

Troy Quigg

2Section of Pediatric Bone Marrow Transplantation and Cellular Therapy, Helen DeVos Children's Hospital, Grand Rapids, United States

D

Deepa Bhojwani

C

Caitlin Elgarten

University of Pennsylvania, Philadelphia