Novel Sulfonium Reagents for the Modular Synthesis of Spiro[2.3]Hexanes and Heteroatom‐Containing Analogues: Synthesis, Application, and Evaluation as Bioisosteres

P Philipp Natho (Department of Pharmacy-Drug Sciences, University of Bari Aldo Moro, Via E. Orabona 4, Bari 70125, Italy) A Annarita Vicenti (Department of Pharmacy‐Drug Sciences University of Bari Aldo Moro Bari Italy) F Fabrizio Mastrolorito (Department of Pharmacy‐Drug Sciences University of Bari “A. Moro” Via E. Orabona 4 Bari 70125 Italy) F Francesca De Franco (Tes Pharma S.r.l. Via Giovine Italia, 1, Solomeo Corciano (PG) 06073 Italy) L Lee Walsh‐Benn (CAS – A division of the American Chemical Society ACS International, Ltd. 2540 Olentangy River Road Columbus Ohio 43202 USA) M Marco Colella (Department of Pharmacy-Drug Sciences, University of Bari Aldo Moro, Via E. Orabona 4, Bari 70125, Italy) E Ernesto Mesto (Department of Earth and Geoenvironmental Sciences University of Bari Aldo Moro Bari Italy) E Emanuela Schingaro (Department of Earth and Geoenvironmental Sciences University of Bari Aldo Moro Bari Italy) O Orazio Nicolotti (Department of Pharmacy‐Drug Sciences University of Bari “A. Moro” Via E. Orabona 4 Bari 70125 Italy) A Antimo Gioiello (Department of Pharmaceutical Sciences University of Perugia Via del Liceo, 1 Perugia 06123 Italy) R Renzo Luisi (Department of Pharmacy-Drug Sciences, University of Bari Aldo Moro, Via E. Orabona 4, Bari 70125, Italy)

Abstract

Abstract Molecular scaffolds with a high fraction of sp 3 ‐hybridized centers have attracted considerable attention in medicinal chemistry as bioisosteres for a wide range of aromatic and nonstrained heterocycles. In particular, strained spiro‐heterocycles have garnered popularity for this purpose, although access to spiro[2.3]hexane analogues is underrepresented. We herein report modular access to nine different spiro[2.3]hexane analogues, including previously underdeveloped 5‐oxa‐1‐azaspiro[2.3]hexane and 1,5‐diazaspiro[2.3]hexane motifs. Our synthetic approach leverages novel cyclobutane‐, oxetane‐, and azetidine‐substituted sulfonium salts, which can undergo Johnson–Corey–Chaykovsky type reactions with alkenes, carbonyls and imines to provide access to the desired spiro[2.3]hexanes. Here, we also report the first comprehensive computational and predictive in silico evaluation of their bioisosteric potential, with validation provided by in vitro experiments.

Article Details

Volume / Issue Vol. 65, Issue 5
Published January 28, 2026
ISSN 1433-7851
Publisher Wiley

Journal Info

Angewandte Chemie International Edition

Wiley

ISSN: 1433-7851 Physical Sciences

Authors (11)

P

Philipp Natho

Department of Pharmacy-Drug Sciences, University of Bari Aldo Moro, Via E. Orabona 4, Bari 70125, Italy

A

Annarita Vicenti

Department of Pharmacy‐Drug Sciences University of Bari Aldo Moro Bari Italy

F

Fabrizio Mastrolorito

Department of Pharmacy‐Drug Sciences University of Bari “A. Moro” Via E. Orabona 4 Bari 70125 Italy

F

Francesca De Franco

Tes Pharma S.r.l. Via Giovine Italia, 1, Solomeo Corciano (PG) 06073 Italy

L

Lee Walsh‐Benn

CAS – A division of the American Chemical Society ACS International, Ltd. 2540 Olentangy River Road Columbus Ohio 43202 USA

M

Marco Colella

Department of Pharmacy-Drug Sciences, University of Bari Aldo Moro, Via E. Orabona 4, Bari 70125, Italy

E

Ernesto Mesto

Department of Earth and Geoenvironmental Sciences University of Bari Aldo Moro Bari Italy

E

Emanuela Schingaro

Department of Earth and Geoenvironmental Sciences University of Bari Aldo Moro Bari Italy

O

Orazio Nicolotti

Department of Pharmacy‐Drug Sciences University of Bari “A. Moro” Via E. Orabona 4 Bari 70125 Italy

A

Antimo Gioiello

Department of Pharmaceutical Sciences University of Perugia Via del Liceo, 1 Perugia 06123 Italy

R

Renzo Luisi

Department of Pharmacy-Drug Sciences, University of Bari Aldo Moro, Via E. Orabona 4, Bari 70125, Italy