Nonwoven fabric coated with cerium oxide nanoparticles for viral inactivation and transmission Inhibition

Abstract

Abstract Studies on virus inactivation by metal nanoparticles indicate that antiviral activity is influenced by the stabilizer on the particle surface. Additionally, cerium oxide nanoparticles stabilized with boric acid (BA-CeO2) exhibit potent antiviral activity. However, previous studies utilized BA-CeO2 dispersed in liquid form and did not fully account for the practical application of antiviral materials in real-world environments. We investigated the antiviral activity of nonwoven fabric coated with BA-CeO2 (NC-NWF). When a medium containing viruses was placed on NC-NWF, the titers of mouse hepatitis virus (MHV), influenza A virus, and feline calicivirus were reduced by > 99% within 2 h. Furthermore, the transmission of MHV was assessed in cages lined with NC-NWF. The cages were divided into two compartments using a mesh and NC-NWF, housing infected and uninfected mice on either side. The results indicated a significantly lower antibody titer against MHV in naïve mice with the NC-NWF partition than in the control partition. Additionally, placing infected mice in NC-NWF cages for 2 h, followed by naïve mice for 24 h, resulted in lower antibody titers against MHV than those in the control fabric. These findings suggest that NC-NWF exhibits antiviral activity and retains efficacy in living environments, such as rearing cages.