Non‐Invasive Diagnosis of Early Colorectal Cancerization via Amplified Sensing of MicroRNA‐21 in NIR‐II Window
Abstract
AbstractAccurate, sensitive, and in situ visualization of aberrant expression level of low‐abundant biomolecules is crucial for early colorectal cancer (CRC) detection ahead of tumor morphology change. However, the clinical used colonoscopy and biopsy methods are invasive and lack of sensitivity at early‐stage of cancerization. Here, an amplified sensing strategy is developed in the second near‐infrared long‐wavelength subregion (NIR‐II‐L, 1500–1900 nm) by integrating DNAzyme‐triggered signal amplification technology and lanthanide‐dye hybrid system. In the early‐stage of CRC, the overexpressed biomarker microRNA‐21 initiates the NIR‐II‐L luminescence ratiometric signal amplification of the CRCsensor. The high sensitivity with a limit of detection (LOD) of 1.26 pm allows non‐invasive visualization of orthotopic colorectal cancerization via rectal administration, which achieves early and accurate in situ diagnosis at 2 weeks ahead of the in vitro histological results. This innovative approach offers a promising tool for early diagnosis and long‐term monitoring of carcinogenesis progression, with potential applications in other cancer‐related biomarkers.
Article Details
Authors (15)
Fuheng Zhai
Department of Chemistry, Laboratory of Advanced Materials, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433, China
Baofeng Yun
Department of Chemistry, Laboratory of Advanced Materials, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433, China
Jiang Ming
State Key Laboratory for Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering
Tianyu Yu
Benhao Li
Laboratory of Advanced Materials, College of Smart Materials and Future Energy (iCOME)
Xiao Liu
Xusheng Wang
Zi‐Han Chen
Department of Chemistry, College of Smart Materials and Future Energy, New Cornerstone Science Laboratory, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials and Ichem Fudan University Shanghai P. R. China
Changfeng Song
Department of Oncology Huadong Hospital Fudan University Shanghai 200040 P. R. China
Mengyao Zhao
Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials and iChem, Shanghai Wusong Laboratory of Materials Science
Wenlin Li
Zhebin Liu
Key Laboratory of Breast Cancer in Shanghai Department of Breast Surgery Fudan University Shanghai Cancer Center Department of Oncology Shanghai Medical College Fudan University Shanghai 200433 P. R. China
Aibin Liang
Jiyu Li
Fan Zhang