Non-invasive imaging of defence responses in plants
Abstract
Abstract Jasmonic and salicylic acids are key hormones involved in plant responses to pests and pathogens. Existing fluorescence-based approaches to imaging plant defence hormones are constrained by the need for external illumination and by autofluorescence of plant tissues, while luminescence-based ones require exogenous substrates. Here, we use jasmonate- and salicylate-responsive promoters to engineer autoluminescent plants that report hormone signalling activity with up to a 53-fold contrast. Using consumer-grade cameras, we image reporter Arabidopsis thaliana and Nicotiana benthamiana plants throughout normal development and in response to pest and pathogen attacks, visualising local and systemic responses. Because the luminescence is self-sustained, these reporters enable non-invasive, substrate-free imaging of defence signalling over extended time courses without specialised equipment.
Article Details
Authors (29)
Anastasia V. Balakireva
Tatiana A. Karataeva
Michael Karampelias
Tatiana Yu Mitiouchkina
Viktor V. Morozov
Jan Macháček
Ekaterina S. Shakhova
Maxim M. Perfilov
Olga A. Belozerova
Sergey I. Kovalchuk
Kseniia A. Palkina
Nikola Drážná
Zuzana Vondráková
Karel Müller
Institute of Experimental Botany of the Czech Academy of Sciences
Tetiana Kalachova
Aubin Fleiss
Josefina Patricia Fernandez-Moreno
Jose M. Alonso
Anna N. Stepanova
Liliia I. Fakhranurova
Nadezhda M. Markina
Dmitry A. Gorbachev
Evgenia N. Bugaeva
Galina M. Delnova
Vladimir V. Choob
Ilia V. Yampolsky
Jan Petrášek
Alexander S. Mishin
Karen S. Sarkisyan