Natural variation of <i>CTS1</i> confers cold tolerance and blast resistance in rice
Abstract
Improving multistress resilience in crops is essential for sustainable agriculture, yet the genetic mechanisms coordinating abiotic and biotic stress responses remain poorly understood. This study identifies CTS1 ( Cold Tolerance at the Seedling stage1 ), encoding the transcription factor OsWRKY74, as a key pleiotropic regulator that mediates both cold tolerance and blast resistance in rice. Through genome-wide association study, we found that CTS1 negatively regulates cold tolerance at the seedling stage. The natural allele CTS1 Hap1 confers greater cold tolerance than CTS1 Hap2 by mildly repressing the expression of CTB2 , thereby maintaining sterol glycoside homeostasis and membrane stability. Furthermore, during Magnaporthe oryzae infection, CTS1 activates the expression of OsHSP90 and upregulates pathogenesis-related genes, thereby enhancing blast resistance. Our findings reveal a dual-function mechanism of CTS1 in stress adaptation and provide valuable alleles for breeding multistress resilient rice varieties.
Article Details
Journal Info
Proceedings of the National Academy of Sciences
National Academy of Sciences
Authors (25)
Wei Ye
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Wendong Ma
Rice Research Institute, Heilongjiang Academy of Agricultural Sciences
Yingxiu Li
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Zhenhua Guo
Rice Research Institute, Heilongjiang Academy of Agricultural Sciences
Shilei Gao
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Haifeng Guo
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Zezhong Yan
Key Laboratory of Pest Monitoring and Green Management, Ministry of Agriculture and Rural Affairs, Joint Laboratory for International Cooperation in Crop Molecular Breeding, Department of Plant Pathology, China Agricultural University
Sijing Shi
Key Laboratory of Pest Monitoring and Green Management, Ministry of Agriculture and Rural Affairs, Joint Laboratory for International Cooperation in Crop Molecular Breeding, Department of Plant Pathology, China Agricultural University
Shichen Han
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Qijin Lou
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Jin Li
Yunsong Gu
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Runbin Su
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Haozhen Wang
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Hafiz Ghulam Nabi
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Yanchen Liu
School of Materials Science and Engineering
Huanhuan Zhu
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Hongliang Zhang
Department of Environmental Science and Engineering, Fudan University
Zhanying Zhang
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Xingming Sun
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Yawen Zeng
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences
Xujun Chen
Key Laboratory of Pest Monitoring and Green Management, Ministry of Agriculture and Rural Affairs, Joint Laboratory for International Cooperation in Crop Molecular Breeding, Department of Plant Pathology, China Agricultural University
Yanjiang Feng
Rice Research Institute, Heilongjiang Academy of Agricultural Sciences
Zichao Li
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University
Jinjie Li
Frontiers Science Center for Molecular Design Breeding, Beijing Key Laboratory of Crop Genetic Improvement, Department of Plant Genetics and Breeding, College of Agronomy and Biotechnology, China Agricultural University