N‐Alkyl Extension in Ionic Molecular Rotors: Facile Tuning of Sensing Modes for Albumin‐Based Host‐Guest Fluorescent Sensors
Abstract
ABSTRACT Establishing a strategy for fine‐tuning of fluorescent sensing modes (e.g., off ↔ on, ratiometric, fluorocolorimetric) in human serum albumin (HSA)‐dye involved supramolecular systems that can tackle the bottleneck for the development of functional fluorescent sensors and sensor arrays in specific practical applications. Here, we propose a simple strategy by extending the N‐alkyl on the pyridinium of triphenylamine diarylethene (TPA‐Ps), a classic ionic molecular rotor skeleton for protein binding and fluorescent sensing, to fulfill the modulation of sensing modes of TPA‐P@HSA. The structural effects on two factors corresponding to sensing modes, the initial fluorescence (TPA‐Ps dispersions) and the final fluorescence (TPA‐P@HSA), have been comprehensively investigated. The utility of the strategy was examined in the applications of fluorescent analysis of urinary albumin, construction of dye@HSA indicator‐displacement assays for the third analytes, fluorescent imaging, and building sensor arrays for classification. Finally, we demonstrated that this strategy can be generalized to other TPA‐P skeleton‐based dyes, resulting in substantially tunable sensing modes in response to HSA.
Article Details
Authors (14)
Caiqiang Liao
State Key Laboratory of Digital Medical Engineering Key Laboratory of Biomedical Engineering of Hainan Province School of Biomedical Engineering Hainan University Sanya Hainan P. R. China
Xinfeng Du
State Key Laboratory of Marine Resource Utilization in South China Sea School of Marine Sciences Hainan University Haikou Hainan P. R. China
Zhang Lin
School of Metallurgy and Environment
Yuting Luo
Department of Oncology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University
Yangyang Yu
Zhaohui Wang
Key Laboratory of Organic Optoelectronics and Molecular Engineering, Department of Chemistry
Jun Peng
State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry
Zhongyong Xu
College of Material Science and Engineering Shenzhen University Shenzhen Guangdong P. R. China
Xianwen Liang
Department of Hepatobiliary & Pancreatic Surgery Hainan General Hospital Haikou Hainan P. R. China
Zhilong Wu
Ya‐Long Wang
State Key Laboratory of Digital Medical Engineering Key Laboratory of Biomedical Engineering of Hainan Province School of Biomedical Engineering Hainan University Sanya Hainan P. R. China
Tianyi Qin
Bin Liu
Ming‐Qiang Zhu
State Key Laboratory of Digital Medical Engineering Key Laboratory of Biomedical Engineering of Hainan Province School of Biomedical Engineering Hainan University Sanya Hainan P. R. China