Myosin-dependent short actin filaments contribute to peripheral widening in developing stereocilia

Abstract

Abstract Stereocilia, the actin-based mechanosensory protrusions of inner ear sensory hair cells, require precise dimensional control for proper mechanotransduction, yet the mechanisms governing actin assembly during development remain unclear. Their size and shape are determined by a stable core of long, parallel, unbranched filamentous (F-) actin. We find that during stereocilia widening, which is a key process for function and stability, newly expressed actin first integrates at the tip, then along the periphery of the core. To understand how actin assembles, we probe for globular (G-) actin, F-actin barbed ends, and pointed ends, and identify a tip-enriched population of short actin filaments. Overexpressing actin increases these filaments at the stereocilia periphery, suggesting a role in widening. In addition, the tip-localized myosins MYO3A/B and MYO15A, essential for normal growth, generate or stabilize short filaments. We propose that these short filaments are intermediates that mature into the long F-actin known to comprise the stereocilia core.