Molecular determinants of thrombosis recurrence risk across venous thromboembolism subtypes

G Gaëlle Munsch (1University of Bordeaux, INSERM, Bordeaux Population Health Research Center, UMR 1219, Bordeaux, France) F Florian Thibord (INSERM UMR 1219, Bordeaux Population Health Research Center, Bordeaux, France (F.T.).) O Ohanna C. Bezerra (4Dalla Lana School of Public Health, University of Toronto, Toronto, ON, Canada) J Jennifer A. Brody A Astrid van Hylckama Vlieg (6Department of Clinical Epidemiology, Leiden University Medical Center, Leiden, The Netherlands) L Lénaïck Gourhant (7University of Brest, INSERM, UMR 1304, Groupe d'étude de la Thrombose de Bretagne Occidentale, Brest, France) M Ming-Huei Chen (2Population Sciences Branch, Division of Intramural Research, National Heart, Lung, and Blood Institute, Framingham, MA) F Floriane Samaria (1University of Bordeaux, INSERM, Bordeaux Population Health Research Center, UMR 1219, Bordeaux, France) M Marine Germain (1University of Bordeaux, INSERM, Bordeaux Population Health Research Center, UMR 1219, Bordeaux, France) I Ilana Caro (1University of Bordeaux, INSERM, Bordeaux Population Health Research Center, UMR 1219, Bordeaux, France) P Pierre Suchon (8Cardiovascular and Nutrition Research Center, INSERM, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, Aix-Marseille University, Marseille, France) R Robert Olaso K Kerri L. Wiggins N Noémie Saut (8Cardiovascular and Nutrition Research Center, INSERM, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, Aix-Marseille University, Marseille, France) C Céline Besse L Louisa Goumidi (8Cardiovascular and Nutrition Research Center, INSERM, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, Aix-Marseille University, Marseille, France) D Delphine Bacq (10Université Paris-Saclay, Commissariat à l'énergie atomique et aux énergies alternatives, Centre National de Recherche en Génomique Humaine, Evry, France) L Laura B. Harrington (11Kaiser Permanente Washington Health Research Institute, Kaiser Permanente Washington, Seattle, WA) A Anne Boland J Joseph Emmerich (14Department of Vascular Medicine, Paris Saint-Joseph Hospital Group, Université Paris Cité, UMR1153, INSERM, Centre de Recherche en Épidémiologie et Statistiques, Paris, France) D David M. Smadja C Catherine A. Lemarié (7University of Brest, INSERM, UMR 1304, Groupe d'étude de la Thrombose de Bretagne Occidentale, Brest, France) S Sven Danckwardt (16Center for Thrombosis and Hemostasis, University Medical Center Mainz, Mainz, Germany) S Stephanie Debette J Jean-François Deleuze H Hélène Jacqmin-Gadda (1University of Bordeaux, INSERM, Bordeaux Population Health Research Center, UMR 1219, Bordeaux, France) M Marc A. Rodger (19Department of Medicine, McGill University, Montreal, QC, Canada) F France Gagnon (4Dalla Lana School of Public Health, University of Toronto, Toronto, ON, Canada) F Frits R. Rosendaal A Andrew D. Johnson N Nicholas L. Smith F Francis Couturaud (Investigation Network on Venous Thrombo-Embolism–French Clinical Research Infrastructure Network, Saint-Étienne, France) P Pierre-Emmanuel Morange D David-Alexandre Trégouët

Abstract

Abstract Venous thromboembolism (VTE) is a frequent (annual incidence of 1-2 per 1000 individuals) and potentially life-threatening (case-fatality rate up to 10%) disease. VTE is associated with serious short-term and long-term complications, including a recurrence rate approaching 20% within 5 years. Anticoagulant therapy, the mainstay of VTE treatment, drastically reduces the risk of early VTE recurrence, but it exposes patients to a substantial risk of bleeding. We analyzed the genomic architecture of VTE recurrence using data from 6355 patients across 8 cohorts (including 1775 recurrences), enriched by subgroup analyses, according to sex and clinical manifestation of first VTE, which led to the identification of 28 molecular markers. Through genome-wide association studies, we identified 1 locus associated with VTE recurrence, GPR149/MME. Among all variants known to be associated with first VTE, KNG1, and FGG were associated with recurrence. Additionally, Mendelian randomization analyses identified 7 proteins as risk factors for recurrence: elevated plasma levels of coagulation factor XI, coagulation factor VIII, von Willebrand factor, histo-blood group ABO system transferase, and Golgi membrane protein 2; and decreased levels of proprotein convertase subtilisin/kexin 9 and pro–interleukin-16. Subgroup analyses revealed 18 molecular determinants associated with VTE recurrence, with notable differences between subgroups. For example, the exonic variant SLC4A1 p.Glu40Lys was significantly associated in patients who experienced pulmonary embolism but showed no effect in those with deep vein thrombosis. These findings emphasize the role of specific genetic loci and protein pathways in influencing VTE recurrence and provide valuable insights into potential therapeutic targets. Further research is needed to clarify the biological mechanisms driving these associations.

Article Details

Journal Blood
Volume / Issue Vol. 146, Issue 19
Published November 06, 2025
Pages 2357-2369
ISSN 0006-4971
Publisher Elsevier BV

Journal Info

Blood

Elsevier BV

ISSN: 0006-4971 Health Sciences

Authors (34)

G

Gaëlle Munsch

1University of Bordeaux, INSERM, Bordeaux Population Health Research Center, UMR 1219, Bordeaux, France

F

Florian Thibord

INSERM UMR 1219, Bordeaux Population Health Research Center, Bordeaux, France (F.T.).

O

Ohanna C. Bezerra

4Dalla Lana School of Public Health, University of Toronto, Toronto, ON, Canada

J

Jennifer A. Brody

A

Astrid van Hylckama Vlieg

6Department of Clinical Epidemiology, Leiden University Medical Center, Leiden, The Netherlands

L

Lénaïck Gourhant

7University of Brest, INSERM, UMR 1304, Groupe d'étude de la Thrombose de Bretagne Occidentale, Brest, France

M

Ming-Huei Chen

2Population Sciences Branch, Division of Intramural Research, National Heart, Lung, and Blood Institute, Framingham, MA

F

Floriane Samaria

1University of Bordeaux, INSERM, Bordeaux Population Health Research Center, UMR 1219, Bordeaux, France

M

Marine Germain

1University of Bordeaux, INSERM, Bordeaux Population Health Research Center, UMR 1219, Bordeaux, France

I

Ilana Caro

1University of Bordeaux, INSERM, Bordeaux Population Health Research Center, UMR 1219, Bordeaux, France

P

Pierre Suchon

8Cardiovascular and Nutrition Research Center, INSERM, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, Aix-Marseille University, Marseille, France

R

Robert Olaso

K

Kerri L. Wiggins

N

Noémie Saut

8Cardiovascular and Nutrition Research Center, INSERM, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, Aix-Marseille University, Marseille, France

C

Céline Besse

L

Louisa Goumidi

8Cardiovascular and Nutrition Research Center, INSERM, Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, Aix-Marseille University, Marseille, France

D

Delphine Bacq

10Université Paris-Saclay, Commissariat à l'énergie atomique et aux énergies alternatives, Centre National de Recherche en Génomique Humaine, Evry, France

L

Laura B. Harrington

11Kaiser Permanente Washington Health Research Institute, Kaiser Permanente Washington, Seattle, WA

A

Anne Boland

J

Joseph Emmerich

14Department of Vascular Medicine, Paris Saint-Joseph Hospital Group, Université Paris Cité, UMR1153, INSERM, Centre de Recherche en Épidémiologie et Statistiques, Paris, France

D

David M. Smadja

C

Catherine A. Lemarié

7University of Brest, INSERM, UMR 1304, Groupe d'étude de la Thrombose de Bretagne Occidentale, Brest, France

S

Sven Danckwardt

16Center for Thrombosis and Hemostasis, University Medical Center Mainz, Mainz, Germany

S

Stephanie Debette

J

Jean-François Deleuze

H

Hélène Jacqmin-Gadda

1University of Bordeaux, INSERM, Bordeaux Population Health Research Center, UMR 1219, Bordeaux, France

M

Marc A. Rodger

19Department of Medicine, McGill University, Montreal, QC, Canada

F

France Gagnon

4Dalla Lana School of Public Health, University of Toronto, Toronto, ON, Canada

F

Frits R. Rosendaal

A

Andrew D. Johnson

N

Nicholas L. Smith

F

Francis Couturaud

Investigation Network on Venous Thrombo-Embolism–French Clinical Research Infrastructure Network, Saint-Étienne, France

P

Pierre-Emmanuel Morange

D

David-Alexandre Trégouët