METHOD DEVELOPMENT AND VALIDATION OF ATAZANAVIR AND RITONAVIR IN THE DOSAGE FORM BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Abstract

The present work describes a validated reverse phase high performance liquid chromatographic method for the estimation of Atazanavir and Ritonavir in the dosage form.The quantification was carried out using Sunfire C18 (250 mm x 4.6 mm), 5µm and mobile phase comprised of Buffer (pH 2.5) and Acetonitrile in proportion of 50: 50 (v/v).The flow rate was 2.0ml/min and the eluent was monitored at 210nm.The selected chromatographic conditions were found to effectively quantitate Atazanavir and Ritonavir at retention time of about  3.5min  and  9min respectively. Linearity were found to be in the range of 29.9792- 449.6885 µg/mL for Atazanavir and 9.9939 - 149.9089µg/ml for Ritonavir.The percentage recoveries of all the drugs were found to be 98.0-102.0%.The proposed method were found to be fast,specific,accurate,precise and reproducible and can be used for the estimation of Atazanavir and Ritonavir drug.