Mesenchymal Stem Cell‐Inspired Microneedle Platform for NIR‐responsive Immunomodulation and Accelerated Chronic Wound Healing
Abstract
Abstract Chronic diabetic wounds present substantial clinical challenges owing to sustained inflammation, compromised vascularization, and inadequate retention of therapeutic medications. Accordingly, motivated by mesenchymal stem cells (MSCs) that actively secrete bioactive exosomes in response to stimuli from the tissue microenvironment, a biomimetic microneedle (MN) platform (MSCi@MN) is created to address these challenges. The MSCi@MN exhibits a dual‐compartment structure composed of MSC‐derived extracellular nanovesicles (NV) conjugated with polydeoxyribonucleotide (PDRN; DNA), referred to as NV‐DNA, encapsulated within dissolvable MN tips, and photothermal‐responsive MXene nanoparticles (MX) incorporated into the base layer for targeted near‐infrared (NIR)‐activated drug delivery. Upon NIR irradiation, MSCi@MN quickly releases NV‐DNA, effectively modifying the immune responses by facilitating anti‐inflammatory M2 macrophage polarization and activating tolerogenic dendritic cells, thereby establishing a regenerative microenvironment. Transcriptomic research has verified that NV‐DNA synergistically promotes angiogenesis, cellular proliferation, and extracellular matrix remodeling by activating complementary molecular pathways. In animal models of diabetes, MSCi@MNs markedly expedite wound repair, diminish inflammation, enhance angiogenesis, and restore skin appendages without systemic adverse effects. This MSC‐inspired approach, which integrates biologically sensitive controlled release with robust immunoregenerative capabilities, has substantial potential for clinical use in chronic wound treatment and regenerative medicine.
Article Details
Authors (22)
Chan Ho Moon
Division of Materials Science and Engineering Hanyang University Seoul Republic of Korea
Hee Gyeong Ko
Department of Biomedical‐Chemical Engineering The Catholic University of Korea Bucheon Gyeonggi‐do 14662 Republic of Korea
Hyun Lee
Seojoon Bang
Division of Materials Science and Engineering Hanyang University Seoul Republic of Korea
Hyeong Seok Kang
Division of Materials Science and Engineering Hanyang University Seoul Republic of Korea
Ju Yeong Gwon
Department of Bioengineering Hanyang University Seoul Republic of Korea
Jong Hwa Seo
Division of Materials Science and Engineering Hanyang University Seoul Republic of Korea
Nayoung Lee
So Won Jeon
Department of Biomedical‐Chemical Engineering The Catholic University of Korea Bucheon Gyeonggi‐do 14662 Republic of Korea
Yun‐A Kim
Department of Biomedical‐Chemical Engineering The Catholic University of Korea Bucheon Gyeonggi‐do 14662 Republic of Korea
Jong Sang Yoon
Department of Biomedical‐Chemical Engineering The Catholic University of Korea Bucheon Gyeonggi‐do 14662 Republic of Korea
Kyung‐Yup Cha
Department of Biomedical Engineering Dongguk University Seoul 04620 Republic of Korea
Min‐Ho Kang
Department of Biomedical‐Chemical Engineering The Catholic University of Korea Bucheon Gyeonggi‐do 14662 Republic of Korea
Dong Yun Lee
Soo‐Hong Lee
Department of Biomedical Engineering Dongguk University Seoul Republic of Korea
Gi Doo Cha
Department of Systems Biotechnology Chung‐Ang University Anseong‐si Gyeonggi‐do Republic of Korea
Kisuk Yang
Division of Bioengineering, College of Life Sciences and Bioengineering Incheon National University Incheon Republic of Korea
Donghyun Lim
Chemical Biology and Therapeutics Science Program
Heemin Kang
Department of Materials Science and Engineering Korea University Seoul South Korea
Su Ryon Shin
Division of Engineering in Medicine Department of Medicine Brigham and Women's Hospital Harvard Medical School Cambridge Massachusetts USA
Han Young Kim
Department of Biomedical‐Chemical Engineering The Catholic University of Korea Bucheon Gyeonggi‐do 14662 Republic of Korea
Hyun‐Do Jung
Division of Materials Science and Engineering Hanyang University Seoul Republic of Korea