Machine learning–enhanced surface-enhanced spectroscopic detection of polycyclic aromatic hydrocarbons in the human placenta

Abstract

The detection and identification of polycyclic aromatic hydrocarbons (PAHs) and their derivatives, polycyclic aromatic compounds (PACs), are essential for environmental and health monitoring, for assessing toxicological exposure and their associated health risks. PAHs/PACs are the most dangerous chemicals found in tobacco smoke, and cigarette use during pregnancy can convey these molecules to the developing fetus through the placenta. This exposure is associated with many negative health outcomes, from premature birth to sudden infant death syndrome and adverse neurodevelopmental disorders. This study demonstrates the use of surface-enhanced Raman and surface-enhanced infrared absorption spectroscopies for direct detection of PAHs/PACs in human placental tissue. We applied two spectroscopy-informed machine learning algorithms, Characteristic Peak Extraction (CaPE) and Characteristic Peak Similarity (CaPSim), to identify the specific PAHs and PACs present in the placenta of women who smoked tobacco cigarettes in pregnancy compared to spectra of the placenta from self-reported nonsmokers. CaPE and CaPSim analysis enabled a clear distinction between these two groups. Independent verification was accomplished by detecting PAH-DNA and PAC-DNA adducts in the smoking group by means of a 32 P-postlabeling assay. These findings highlight the effectiveness of combining surface-enhanced spectroscopies with informed ML analysis for the streamlined detection of hazardous environmental compounds in human tissues, suggesting broader applications in clinical diagnostics and public health surveillance.