Long Single‐Stranded DNA with Site‐Specific Modifications to Enable Single‐Base‐Resolution Epigenetics
Abstract
Abstract We report a method for synthesizing site‐specifically modified long single‐stranded DNA (ssDNA) with high purity and fidelity. With the successful synthesis of long ssDNA carrying different 5‐methylcytosine (5mC) patterns, single‐base‐resolution analysis of 5mC epigenetic dynamics at specific genomic loci were enabled in mammalian cells. The results revealed that the density of 5mC sites synergistically correlated with their maintenance. This study opens new avenues for precise epigenetic manipulation and therapeutic applications.
Article Details
Authors (16)
Chenyou Zhu
Department of Chemistry
Yin Xu
Ziyang Hao
School of Pharmaceutical Sciences
Yuan Zhuang
Department of Chemistry
Rui Xu
College & Hospital of Stomatology
Yufan Pan
Department of Chemistry
Jiezhong Shi
Engineering Research Center of Advanced Rare Earth Materials (Ministry of Education) Department of Chemistry Tsinghua University Beijing 100084 P.R. China
Yujie Li
Engineering Research Center of Advanced Rare Earth Materials (Ministry of Education), Department of Chemistry
Yifan Jiang
Micro−Nano Engineering Sciences Research Center, School of Mechanical Engineering
Tianran Wang
Yuhe R. Yang
CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety CAS Key Laboratory of Nanosystem and Hierarchical Fabrication CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology Beijing P.R. China
Huajie Liu
School of Chemical Science and Engineering Shanghai Research Institute for Intelligent Autonomous Systems Key Laboratory of Advanced Civil Engineering Materials of Ministry of Education Tongji University Shanghai 200092 P.R. China
Dali Han
Yuanchen Dong
CAS Key Laboratory of Colloid Interface and Chemical Thermodynamics, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry
Chuan He
Dongsheng Liu
Department of Chemistry