Large-scale experimental investigation of biotreated sand column using different grouting pipe configurations

C Chunyan Wang (Department of Oncology, School of Medicine and Public Health, University of Wisconsin) Z Zhikang Wei (Key Laboratory of Chemical Biology and Traditional Chinese Medicine (Ministry of Educational of China), Key Laboratory of the Assembly and Application of Organic Functional Molecules of Hunan Province, College of Chemistry and Chemical Engineering) J Jinquan Shi (Energy Sciences Institute) X Xiang He (Anhui iAmetal New Energy Technology Co.,Ltd)

Abstract

Microbially induced carbonate precipitation (MICP) has considerable potential for applications such as soil improvement, erosion control, and heavy metal remediation. However, the extent to which grouting pipe configuration affects treatment performance remains unclear. To address this gap, this study investigated the effects of grouting pipe configuration on the reinforcement behaviour of large-scale biotreated sand columns. Large-scale model tests were conducted using conventional vertical pipes and a newly proposed spiral pipe under different grouting procedures. Calcium carbonate content, unconfined compressive strength, penetration resistance, and microscopic characteristics were analysed to evaluate reinforcement range, spatial uniformity, and treatment depth. The results show that pipe configuration plays a key role in controlling calcium carbonate distribution and reinforcement performance. The one-pipe-injection-extraction configuration produced a limited effective reinforcement radius of about 0.09 m, whereas both the three-injection-one-extraction and spiral-injection-one-extraction configurations increased this value to about 0.15 m. Compared with conventional vertical pipes, the spiral configuration produced a more uniform distribution of calcium carbonate content and strength by reducing local enrichment and weakly treated zones. For the 1.5 m-high specimen, relatively uniform reinforcement was mainly achieved within the upper 0.6 m under the present pressure level. This study provides a practical pipe-layout strategy for improving the uniformity of field-scale MICP treatment and offers useful guidance for the design of bio-grouting systems.

Article Details

Journal PLoS ONE
Volume / Issue Vol. 21, Issue 5
Published May 26, 2026
Pages e0349797
ISSN 1932-6203
Publisher Public Library of Science

Journal Info

PLoS ONE

Public Library of Science

ISSN: 1932-6203 Open Access Health Sciences

Authors (4)

C

Chunyan Wang

Department of Oncology, School of Medicine and Public Health, University of Wisconsin

Z

Zhikang Wei

Key Laboratory of Chemical Biology and Traditional Chinese Medicine (Ministry of Educational of China), Key Laboratory of the Assembly and Application of Organic Functional Molecules of Hunan Province, College of Chemistry and Chemical Engineering

J

Jinquan Shi

Energy Sciences Institute

X

Xiang He

Anhui iAmetal New Energy Technology Co.,Ltd