Isoflavonoids from Iris albicans as a carbon source to enhance the anti-aging potential of lactic acid bacteria-derived postbiotics

Abstract

Abstract Dietary strategies that limit protein glycation can help slow aging and prevent age‑related diseases by reducing the accumulation of advanced glycation end products (AGEs). This study evaluated the antioxidant, antiglycation, and prebiotic potential of postbiotics produced by lactic acid bacteria using Iris albicans extract and its major isoflavonoids as carbon sources. Antioxidant activity (DPPH, ABTS, FRAP), prebiotic potential (A preb activity scores), and antiglycation effects were assessed via fructosamine formation, oxidative stress markers, protein aggregation, and AGE‑RAGE inhibition assays. Among all tested postbiotics, Limosilactobacillus reuteri MSD37 showed the highest antioxidant and antiglycation activities, especially when cultured with I. albicans extract or irilone. This postbiotic effectively preserved thiol groups, reduced protein oxidation, and inhibited AGE‑RAGE interactions. Moreover, L. reuteri MSD37 postbiotic enriched with I. albicans extract exhibited notably high prebiotic activity toward probiotic strains. Overall, I. albicans represents a promising carbon source for producing anti‑aging postbiotics, highlighting the potential of L. reuteri MSD37 as a gerobiotic candidate for promoting healthy aging.