<i>SHOOT GRAVITROPISM 9</i> links sensory timing to the initial lateral root growth angle

S Sophie Zoe Farkas (Institute of Biology II, Chair of Molecular Plant Physiology, University of Freiburg) F Federico Grippo (Institute of Biology II, Chair of Molecular Plant Physiology, University of Freiburg) D Denisa Oulehlová (Department of Experimental Plant Biology, Faculty of Science, Charles University) A Alberto González-Delgado (Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid—Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Científicas (CSIC)) S Seinab Noura (Institute of Biology II, Chair of Molecular Plant Physiology, University of Freiburg) S Sima Molazeinali (Institute of Biology II, Chair of Molecular Plant Physiology, University of Freiburg) K Krzysztof Wabnik (Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid—Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Científicas (CSIC)) M Matyáš Fendrych (Institute of Experimental Botany of the Czech Academy of Sciences) S Sascha Waidmann (Institute of Biology II, Chair of Molecular Plant Physiology, University of Freiburg) J Jürgen Kleine-Vehn (Institute of Biology II, Chair of Molecular Plant Physiology, University of Freiburg)

Abstract

Plants strategically respond to gravity and actively set defined growth angles. These gravitropic set-point angles (GSAs) shape the width and depth of the root system, determining soil exploration for water and nutrient uptake. Despite its physiological importance, the concept by which lateral roots establish their growth angle has remained largely unclear. Here, we show that temporal regulation of gravity responsiveness within a defined developmental phase sets the initial GSA in lateral roots. Stage-specific transcriptome profiling revealed that SHOOT GRAVITROPISM 9 ( SGR9 ) is upregulated in emerged lateral roots as they first respond to gravity. Loss of SGR9 slows amyloplast sedimentation and delays the PIN3-dependent establishment of asymmetric auxin distribution. Notably, the delay in the gravity response alters, rather than merely postpones, the initial stable growth angle. Our findings reveal that a developmental time window enables transient sensory dynamics to be translated into stable initial root growth angles, illustrating how developmental timing can convert short-lived signals into lasting form.

Article Details

Volume / Issue Vol. 123, Issue 30
Published July 28, 2026
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (10)

S

Sophie Zoe Farkas

Institute of Biology II, Chair of Molecular Plant Physiology, University of Freiburg

F

Federico Grippo

Institute of Biology II, Chair of Molecular Plant Physiology, University of Freiburg

D

Denisa Oulehlová

Department of Experimental Plant Biology, Faculty of Science, Charles University

A

Alberto González-Delgado

Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid—Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Científicas (CSIC)

S

Seinab Noura

Institute of Biology II, Chair of Molecular Plant Physiology, University of Freiburg

S

Sima Molazeinali

Institute of Biology II, Chair of Molecular Plant Physiology, University of Freiburg

K

Krzysztof Wabnik

Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid—Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Científicas (CSIC)

M

Matyáš Fendrych

Institute of Experimental Botany of the Czech Academy of Sciences

S

Sascha Waidmann

Institute of Biology II, Chair of Molecular Plant Physiology, University of Freiburg

J

Jürgen Kleine-Vehn

Institute of Biology II, Chair of Molecular Plant Physiology, University of Freiburg