Investigation of the functional hot-spot residues of an enzyme by real-time monitoring of the enzymatic reaction using NMR and computational approaches

T Toshihiko Sugiki T Tomoki Yoshida M Masaki Tsukamoto K Koichiro Miyanishi A Akinori Kagawa N Natsuko Miura T Tomoto Ura J Jun Fukazawa Y Yuko Hatanaka T Tsuyoshi Murata T Toshimichi Fujiwara (Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Osaka, Suita 565-0871, Japan) M Masahiro Kitagawa Y Yasushi Morita K Kumiko Sakai-Kato Y Yoichi Takakusagi N Nobutada Tanaka M Makoto Negoro