Interactions among nutrients govern the global grassland biomass–precipitation relationship

P Philip A. Fay (United States Department of Agriculture, Agricultural Research Service, Grassland, Soil, and Water Lab) L Laureano A. Gherardi (Department of Environmental Sciences, Policy, and Management, University of California) L Laura Yahdjian (Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura-Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Ecología, Facultad de Agronomía, Universidad de Buenos Aires) P Peter B. Adler (Department of Wildland Resources and the Ecology Center, Utah State University) J Jonathan D. Bakker (School of Environmental and Forest Sciences, University of Washington) S Siddharth Bharath (Department of Ecology, Evolution, and Behavior, University of Minnesota) E Elizabeth T. Borer (Department of Ecology, Evolution, and Behavior, University of Minnesota) W W. Stanley Harpole (German Centre for Integrative Biodiversity Research, Halle-Jena-Leipzig) E Erika Hersch-Green (Department of Biological Sciences, Michigan Technological University) T Travis E. Huxman (Department of Ecology and Evolutionary Biology, University of California) A Andrew S. MacDougall (Department of Integrative Biology, University of Guelph) A Anita C. Risch (Swiss Federal Institute for Forest, Snow and Landscape Research WSL) E Eric W. Seabloom (Department of Ecology, Evolution, and Behavior, University of Minnesota) S Sumanta Bagchi (Centre for Ecological Sciences, Indian Institute of Science) I Isabel C. Barrio (Faculty of Environmental and Forest Sciences, Agricultural University of Iceland) L Lori Biederman (Department of Ecology, Evolution, and Organismal Biology, Iowa State University) Y Yvonne M. Buckley (Co-Centre for Climate + Biodiversity + Water, School of Natural Sciences, Trinity College Dublin) M Miguel N. Bugalho (Center for Applied Ecology “Prof. Baeta Neves”-Research Network in Biodiversity and Evolutionary Biology, School of Agriculture, University of Lisbon) M Maria C. Caldeira (Forest Research Centre, Associate Laboratory TERRA, School of Agriculture, University of Lisbon) J Jane A. Catford (Department of Geography, King’s College London) Q Qingqing Chen E Elsa E. Cleland (Ecology, Behavior & Evolution Department, University of California San Diego) S Scott L. Collins P Pedro Daleo (Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata-Consejo Nacional de Investigaciones Científicas y Técnicas) C Christopher R. Dickman (Desert Ecology Research Group, School of Life and Environmental Sciences, The University of Sydney) I Ian Donohue (Zoology, School of Natural Sciences, Trinity College Dublin) M Mary E. DuPre (MPG Ranch) N Nico Eisenhauer A Anu Eskelinen N Nicole Hagenah Y Yann Hautier R Robert W. Heckman (Department of Biology, University of North Carolina) I Ingibjörg S. Jónsdóttir J Johannes M. H. Knops (Department of Health and Environmental Sciences, Xi’an Jiaotong-Liverpool University) R Ramesh Laungani (Department of Environmental Science and Policy, Marist College) J Jason P. Martina (Department of Biology, Texas State University) R Rebecca L. McCulley (Department of Plant and Soil Sciences, University of Kentucky) J John W. Morgan (Department of Environment and Genetics, La Trobe University) H Harry Olde Venterink (Department of Biology-Wildness, Biodiversity, and Ecosystems Under Change, Vrije Universiteit Brussel) P Pablo L. Peri S Sally A. Power (Hawkesbury Institute for the Environment, Western Sydney University) X Xavier Raynaud (Sorbonne Université, Université de Paris- Cité, Université Paris-Est Créteil, Institut de Recherche pour le Développement, Centre National de la Recherche Scientifique, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Institut d'Ecologie et des Sciences de l'Envrionnement de Paris) Z Zhengwei Ren (College of Ecology, Lanzhou University) C Christiane Roscher (German Centre for Integrative Biodiversity Research, Halle-Jena-Leipzig) M Melinda D. Smith M Marie Spohn (Department of Soil and Environment, Swedish University of Agricultural Sciences) C Carly J. Stevens (Lancaster Environment Centre, Lancaster University) M Michelle J. Tedder (Centre for Functional Biodiversity, School of Life Sciences, University of KwaZulu-Natal) R Risto Virtanen G Glenda M. Wardle (School of Life and Environmental Sciences, ARC Training Centre in Data Analytics for Resources and Environments, The University of Sydney) G George R. Wheeler (Department of Biological Sciences, Michigan Technological University)

Abstract

Ecosystems are experiencing changing global patterns of mean annual precipitation (MAP) and enrichment with multiple nutrients that potentially colimit plant biomass production. In grasslands, mean aboveground plant biomass is closely related to MAP, but how this relationship changes after enrichment with multiple nutrients remains unclear. We hypothesized the global biomass–MAP relationship becomes steeper with an increasing number of added nutrients, with increases in steepness corresponding to the form of interaction among added nutrients and with increased mediation by changes in plant community diversity. We measured aboveground plant biomass production and species diversity in 71 grasslands on six continents representing the global span of grassland MAP, diversity, management, and soils. We fertilized all sites with nitrogen, phosphorus, and potassium with micronutrients in all combinations to identify which nutrients limited biomass at each site. As hypothesized, fertilizing with one, two, or three nutrients progressively steepened the global biomass–MAP relationship. The magnitude of the increase in steepness corresponded to whether sites were not limited by nitrogen or phosphorus, were limited by either one, or were colimited by both in additive, or synergistic forms. Unexpectedly, we found only weak evidence for mediation of biomass–MAP relationships by plant community diversity because relationships of species richness, evenness, and beta diversity to MAP and to biomass were weak or opposing. Site-level properties including baseline biomass production, soils, and management explained little variation in biomass–MAP relationships. These findings reveal multiple nutrient colimitation as a defining feature of the global grassland biomass–MAP relationship.

Article Details

Volume / Issue Vol. 122, Issue 15
Published April 15, 2025
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (51)

P

Philip A. Fay

United States Department of Agriculture, Agricultural Research Service, Grassland, Soil, and Water Lab

L

Laureano A. Gherardi

Department of Environmental Sciences, Policy, and Management, University of California

L

Laura Yahdjian

Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura-Consejo Nacional de Investigaciones Científicas y Técnicas, Cátedra de Ecología, Facultad de Agronomía, Universidad de Buenos Aires

P

Peter B. Adler

Department of Wildland Resources and the Ecology Center, Utah State University

J

Jonathan D. Bakker

School of Environmental and Forest Sciences, University of Washington

S

Siddharth Bharath

Department of Ecology, Evolution, and Behavior, University of Minnesota

E

Elizabeth T. Borer

Department of Ecology, Evolution, and Behavior, University of Minnesota

W

W. Stanley Harpole

German Centre for Integrative Biodiversity Research, Halle-Jena-Leipzig

E

Erika Hersch-Green

Department of Biological Sciences, Michigan Technological University

T

Travis E. Huxman

Department of Ecology and Evolutionary Biology, University of California

A

Andrew S. MacDougall

Department of Integrative Biology, University of Guelph

A

Anita C. Risch

Swiss Federal Institute for Forest, Snow and Landscape Research WSL

E

Eric W. Seabloom

Department of Ecology, Evolution, and Behavior, University of Minnesota

S

Sumanta Bagchi

Centre for Ecological Sciences, Indian Institute of Science

I

Isabel C. Barrio

Faculty of Environmental and Forest Sciences, Agricultural University of Iceland

L

Lori Biederman

Department of Ecology, Evolution, and Organismal Biology, Iowa State University

Y

Yvonne M. Buckley

Co-Centre for Climate + Biodiversity + Water, School of Natural Sciences, Trinity College Dublin

M

Miguel N. Bugalho

Center for Applied Ecology “Prof. Baeta Neves”-Research Network in Biodiversity and Evolutionary Biology, School of Agriculture, University of Lisbon

M

Maria C. Caldeira

Forest Research Centre, Associate Laboratory TERRA, School of Agriculture, University of Lisbon

J

Jane A. Catford

Department of Geography, King’s College London

Q

Qingqing Chen

E

Elsa E. Cleland

Ecology, Behavior & Evolution Department, University of California San Diego

S

Scott L. Collins

P

Pedro Daleo

Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata-Consejo Nacional de Investigaciones Científicas y Técnicas

C

Christopher R. Dickman

Desert Ecology Research Group, School of Life and Environmental Sciences, The University of Sydney

I

Ian Donohue

Zoology, School of Natural Sciences, Trinity College Dublin

M

Mary E. DuPre

MPG Ranch

N

Nico Eisenhauer

A

Anu Eskelinen

N

Nicole Hagenah

Y

Yann Hautier

R

Robert W. Heckman

Department of Biology, University of North Carolina

I

Ingibjörg S. Jónsdóttir

J

Johannes M. H. Knops

Department of Health and Environmental Sciences, Xi’an Jiaotong-Liverpool University

R

Ramesh Laungani

Department of Environmental Science and Policy, Marist College

J

Jason P. Martina

Department of Biology, Texas State University

R

Rebecca L. McCulley

Department of Plant and Soil Sciences, University of Kentucky

J

John W. Morgan

Department of Environment and Genetics, La Trobe University

H

Harry Olde Venterink

Department of Biology-Wildness, Biodiversity, and Ecosystems Under Change, Vrije Universiteit Brussel

P

Pablo L. Peri

S

Sally A. Power

Hawkesbury Institute for the Environment, Western Sydney University

X

Xavier Raynaud

Sorbonne Université, Université de Paris- Cité, Université Paris-Est Créteil, Institut de Recherche pour le Développement, Centre National de la Recherche Scientifique, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Institut d'Ecologie et des Sciences de l'Envrionnement de Paris

Z

Zhengwei Ren

College of Ecology, Lanzhou University

C

Christiane Roscher

German Centre for Integrative Biodiversity Research, Halle-Jena-Leipzig

M

Melinda D. Smith

M

Marie Spohn

Department of Soil and Environment, Swedish University of Agricultural Sciences

C

Carly J. Stevens

Lancaster Environment Centre, Lancaster University

M

Michelle J. Tedder

Centre for Functional Biodiversity, School of Life Sciences, University of KwaZulu-Natal

R

Risto Virtanen

G

Glenda M. Wardle

School of Life and Environmental Sciences, ARC Training Centre in Data Analytics for Resources and Environments, The University of Sydney

G

George R. Wheeler

Department of Biological Sciences, Michigan Technological University