Integrative transcriptomic and metabolomic analyses provide insights into the mechanism of autotoxicity of Pugionium cornutum (L.) Gaertn

Abstract

Pugionium cornutum (L.) Gaertn., a functional vegetable with dual health benefits, faces significant constraints in sustainable industrial development due to autotoxicity-induced continuous cropping obstacles. This study investigates the molecular regulatory mechanisms underlying the response of Pugionium cornutum (L.) Gaertn. to phthalic acid, a representative autotoxic substance, through integrated transcriptomic and metabolomic analyses under varying phthalic acid concentrations. The results revealed that phthalic acid stress significantly altered the abundance of 892 metabolites. Critical metabolites including MG(16:0/0:0/0:0)[rac] and 6-hydroxysphingosine were found to modulate differentially expressed genes (DEGs) involved in key pathways such as flavonoid biosynthesis, tropane/piperidine/pyridine alkaloid biosynthesis, glucosinolate metabolism, and ABC transporter activity. Comparative analysis demonstrated more pronounced molecular responses under high-concentration phthalic acid stress (10 mmol/L) compared to low-concentration treatment (0.1 m mmol/L), indicating intensified phytotoxic effects at elevated autotoxin levels. These findings provide novel insights into the autotoxicity response mechanisms of Pugionium cornutum (L.) Gaertn.and establish a theoretical foundation for developing sustainable cultivation strategies for this species.