Increasing TET Expression and 5‐Hydroxymethylcytosine Formation by a Carbocyclic 5‐Aza‐2′‐deoxy‐cytidine Antimetabolite
Abstract
ABSTRACT Ten‐eleven translocation (TET) enzymes are critical epigenetic regulators, which oxidize the methylated cytosine nucleobase 5‐methyl‐dC (mdC) in the genome to 5‐hydroxymethyl‐dC (hmdC) in an α‐ketoglutarate‐dependent manner. Because the presence of mdC in the promoter region of a given gene silences its expression, this oxidation goes in hand with the reactivation of such silenced genes. In different highly aggressive cancers such as acute myeloid leukemia (AML) and glioblastoma, loss of TET enzyme function, and therefore reduced hmdC levels pave the way for tumor development. Impairment of TET activity can occur through metabolic inhibition, through loss‐of‐function mutations in TET genes themselves, and finally through suppression of TET‐expression via epigenetic silencing. Reactivation of TET enzyme expression represents a major aim of epigenetic cancer therapy. Here we show that the carbocyclic antimetabolite 5‐aza‐2′deoxycytidine (cAzadC), which is supposed to suppress the methylation of DNA during replication, leads to a substantial increase of TET2 expression and strongly increasing hmdC levels. We show that the treatment with cAzadC goes in hand with the broad reactivation of the cellular antitumor responses. With patient‐derived xenograft AML‐mouse models, we show that this translates into a strongly improved anticancer effect in vivo.
Article Details
Authors (18)
Maike Däther
Institute for Chemical Epigenetics and Center for Nucleic Acid Therapies Department of Chemistry LMU Munich Munich Germany
Elsa Peev
Institute for Chemical Epigenetics and Center for Nucleic Acid Therapies Department of Chemistry LMU Munich Munich Germany
Annika Fröhlich
Research Unit Apoptosis in Hematopoietic Stem Cells Helmholtz Munich German Research Center for Environmental Health (HMGU) Munich Germany
Binje Vick
Sogol Fatourechi
Institute for Chemical Epigenetics and Center for Nucleic Acid Therapies Department of Chemistry LMU Munich Munich Germany
Gilles Gasparoni
Matthias Heiß
Corinna C. Pleintinger
Institute for Chemical Epigenetics and Center for Nucleic Acid Therapies Department of Chemistry LMU Munich Munich Germany
Emmanuel Asu Bisong
Institute for Chemical Epigenetics and Center for Nucleic Acid Therapies Department of Chemistry LMU Munich Munich Germany
Hans Hurmiz
Institute for Chemical Epigenetics and Center for Nucleic Acid Therapies Department of Chemistry LMU Munich Munich Germany
Davide Guglielminotti
Institute for Chemical Epigenetics and Center for Nucleic Acid Therapies Department of Chemistry LMU Munich Munich Germany
Yasmin V. Gärtner
Institute for Chemical Epigenetics and Center for Nucleic Acid Therapies Department of Chemistry LMU Munich Munich Germany
Tina Aumer
Institute for Chemical Epigenetics and Center for Nucleic Acid Therapies Department of Chemistry LMU Munich Munich Germany
Karsten Spiekermann
Ludwig Maximilian University Hospital, Munich, Germany
Jörn Walter
Irmela Jeremias
Franziska R. Traube
Institute for Chemical Epigenetics and Center for Nucleic Acid Therapies Department of Chemistry LMU Munich Munich Germany
Thomas Carell