HIV-1 envelope trimer vaccine induces sex-associated differences in antibody responses: a phase 1 clinical trial

E Emma I. M. M. Reiss K Karlijn van der Straten L Laura T. M. Graus M Marloes Grobben K Kilian E. Vlaming A Annelou I. P. van der Veen M Marinus H. Liesdek G Gabriel Ozorowski M Martin Corcoran H Hongmei Gao (Duke Human Vaccine Institute, Duke University Medical Center) K Kelli M. Greene N Nicole L. Yates S Sheetal Sawant G Gius Kerster J Judith A. Burger S Stella Schonherr H Hannah M. Cheeseman A Abbey Evans L Leon R. McFarlane A Andy S. Tran J Jonathan L. Torres (Department of Integrative Structural and Computational Biology, The Scripps Research Institute) R Ryan N. Lin G Gyunghee Jo M Monica Tolazzi P Philipp Mundsperger D Dietmar Katinger A Albert Cupo J John P. Moore R Rob Hurks L Liffert Vogt M Maarten R. Soeters N Neeltje A. Kootstra G Gabriella Scarlatti G Georgia D. Tomaras D David C. Montefiori (Duke Human Vaccine Institute, Duke University Medical Center) G Gunilla B. Karlsson Hedestam A Andrew B. Ward M Michelle Klouwens M Menno D. de Jong J Jan M. Prins M Mathieu Claireaux T Teunis B. H. Geijtenbeek R Robin J. Shattock M Marit J. van Gils (Department of Medical Microbiology and Infection Prevention, Amsterdam Infection and Immunity Institute, Amsterdam University Medical Center, University of Amsterdam) R Rogier W. Sanders (Department of Medical Microbiology and Infection Prevention, Amsterdam Infection and Immunity Institute, Amsterdam University Medical Center, University of Amsterdam) G Godelieve J. de Bree

Abstract

Abstract A protective vaccine will be the most powerful instrument to reduce HIV-1 infections worldwide and help bring about a lasting end to the AIDS epidemic. The single centre, randomised, open-label, uncontrolled, phase 1 ACTHIVE-001 clinical trial (NCT03961438) aims to assess the safety and immunogenicity of the ConM SOSIP.v7 native-like trimer protein vaccine, based on an HIV-1 group M consensus sequence, in HIV-negative adults. Twenty-four individuals were enrolled to receive three dosages of ConM SOSIP.v7 protein vaccine in a liposome formulation containing a high dose of the TLR4-agonist MPLA. The primary outcome is vaccine reactogenicity, whereas the main secondary outcome is binding and neutralising antibody responses. Overall, the vaccine is safe and well-tolerated. Furthermore, the vaccine elicits robust strain-specific binding and neutralising antibody responses in nearly all vaccinees. Post-hoc exploratory analyses demonstrate that female-born participants have 22- and 6-fold higher neutralisation titres after the second and third vaccination, respectively. The vaccine adjuvant induces higher levels of IL-6 secretion from in vitro cultured monocytes from female compared to male participants, providing a possible mechanistic explanation for the sex-based differences. Our study highlights the need to take sex-based differences into consideration when assessing HIV-1 vaccine candidates and adjuvants.

Article Details

Volume / Issue Vol. 16, Issue 1
Published November 21, 2025
ISSN 2041-1723
Publisher Nature Portfolio

Journal Info

Nature Communications

Nature Portfolio

ISSN: 2041-1723 Open Access Life Sciences

Authors (46)

E

Emma I. M. M. Reiss

K

Karlijn van der Straten

L

Laura T. M. Graus

M

Marloes Grobben

K

Kilian E. Vlaming

A

Annelou I. P. van der Veen

M

Marinus H. Liesdek

G

Gabriel Ozorowski

M

Martin Corcoran

H

Hongmei Gao

Duke Human Vaccine Institute, Duke University Medical Center

K

Kelli M. Greene

N

Nicole L. Yates

S

Sheetal Sawant

G

Gius Kerster

J

Judith A. Burger

S

Stella Schonherr

H

Hannah M. Cheeseman

A

Abbey Evans

L

Leon R. McFarlane

A

Andy S. Tran

J

Jonathan L. Torres

Department of Integrative Structural and Computational Biology, The Scripps Research Institute

R

Ryan N. Lin

G

Gyunghee Jo

M

Monica Tolazzi

P

Philipp Mundsperger

D

Dietmar Katinger

A

Albert Cupo

J

John P. Moore

R

Rob Hurks

L

Liffert Vogt

M

Maarten R. Soeters

N

Neeltje A. Kootstra

G

Gabriella Scarlatti

G

Georgia D. Tomaras

D

David C. Montefiori

Duke Human Vaccine Institute, Duke University Medical Center

G

Gunilla B. Karlsson Hedestam

A

Andrew B. Ward

M

Michelle Klouwens

M

Menno D. de Jong

J

Jan M. Prins

M

Mathieu Claireaux

T

Teunis B. H. Geijtenbeek

R

Robin J. Shattock

M

Marit J. van Gils

Department of Medical Microbiology and Infection Prevention, Amsterdam Infection and Immunity Institute, Amsterdam University Medical Center, University of Amsterdam

R

Rogier W. Sanders

Department of Medical Microbiology and Infection Prevention, Amsterdam Infection and Immunity Institute, Amsterdam University Medical Center, University of Amsterdam

G

Godelieve J. de Bree