High Temporal‐Resolution Imaging Single Immune Cell Migration in Vivo by Ultrabright NIR‐II Organic Probes
Abstract
ABSTRACT Given the implications of immune cell migration and cellular interaction in immune‐oncology, infectious and inflammatory diseases, there is a long‐standing interest in understanding, predicting, and ultimately manipulating immune cell migration therapeutically. However, real‐time non‐invasive in vivo immune cell tracking is considerably more challenging than static imaging, particularly when imaging highly motile cells. Here we introduce high‐brightness second near‐infrared (NIR‐II, 1000–1700 nm) window organic probes (DCPDs, brightness > 5000 M −1 cm −1 in aqueous solution), enabling non‐invasive high‐speed single immune cell imaging in the brain with a temporal resolution up to ∼50 fps. The neutrophil migration patterns in the stroke mouse brain and their interactions with infused therapeutic mesenchymal stem cells can be profiled with real temporal dynamics. This advancement facilitates fundamental studies on understanding complex cellular functions in vivo, paving the way for investigating the effect of single‐cell temporal dynamics on immune responses or disease‐related biological processes.
Article Details
Authors (11)
Ying Chen
Yang Yang
Baofeng Yun
Department of Chemistry, Laboratory of Advanced Materials, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433, China
Zuyang He
Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials and iChem, Shanghai Wusong Laboratory of Materials Science
Hongxin Zhang
Department of Chemistry, Laboratory of Advanced Materials, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200433, China
Qunying Jiang
College of Energy Materials and Chemistry Inner Mongolia University Hohhot P. R. China
Wenlin Li
Aibin Liang
Lixin Wang
Department of Chemistry
Bin‐Zhi Qian
Human Phenome Institute Zhangjiang Fudan International Innovation Center Fudan University Shanghai P. R. China
Fan Zhang