High-intensity interval training differentially modulates acute BDNF and cognitive responses in young adult males: a randomized crossover trial

Abstract

Abstract Exercise is widely recognized for its beneficial effects on brain health, yet the extent to which exercise intensity modulates acute neurochemical and cognitive responses remains unclear. Particularly, the role of exercise intensity in shaping brain-derived neurotrophic factor (BDNF), lactate responses, and executive function requires further investigation. This study compared the acute effects of low-intensity continuous training (LICT), moderate-intensity continuous training (MICT), high-intensity interval training (HIIT), and a resting control condition (CTRL) on BDNF levels, blood lactate concentration, and cognitive responses in healthy young adult males. Twelve healthy young adult males completed LICT, MICT, HIIT, and the control condition using a randomized crossover design with a 7-day washout period. Serum BDNF, blood lactate concentration, and executive function assessed by the Stroop Test were measured before and immediately after each experimental condition. HIIT induced significantly greater post-exercise increases in BDNF and lactate compared with all other conditions, while MICT elicited moderate elevations relative to LICT and rest. Lactate responses increased progressively with exercise intensity. Improvements in executive function were observed exclusively following HIIT, reflected by significantly faster Stroop Test completion times. HIIT produced concurrent elevations in lactate and serum BDNF together with improved executive function performance. HIIT may represent an effective acute stimulus for cognitive benefits, with potential relevance for exercise approaches aimed at supporting brain health via neurotrophic signaling. Trial registration: The study was retrospectively registered on ClinicalTrials.gov (identifier: NCT07137611; https://clinicaltrials.gov/study/NCT07137611 ) on 22 August 2025.