HiBC: a publicly available collection of bacterial strains isolated from the human gut
Abstract
Abstract Numerous bacteria in the human gut microbiome remain unknown and/or have yet to be cultured. While collections of human gut bacteria have been published, few strains are accessible to the scientific community. We have therefore created a publicly available collection of bacterial strains isolated from the human gut. The Human intestinal Bacteria Collection (HiBC) ( https://www.hibc.rwth-aachen.de ) contains 340 strains representing 198 species within 29 families and 7 phyla, of which 29 previously unknown species are taxonomically described and named. These included two butyrate-producing species of Faecalibacterium and new dominant species associated with health and inflammatory bowel disease, Ruminococcoides intestinale and Blautia intestinihominis , respectively. Plasmids were prolific within the HiBC isolates, with almost half (46%) of strains containing plasmids, with a maximum of six within a strain. This included a broadly occurring plasmid (pBAC) that exists in three diverse forms across Bacteroidales species. Megaplasmids were identified within two strains, the pMMCAT megaplasmid is globally present within multiple Bacteroidales species. This collection of easily searchable and publicly available gut bacterial isolates will facilitate functional studies of the gut microbiome.
Article Details
Authors (44)
Thomas C. A. Hitch
Johannes M. Masson
Charlie Pauvert
Johanna Bosch
Selina Nüchtern
Nicole S. Treichel
Marko Baloh
Soheila Razavi
Afrizal Afrizal
Ntana Kousetzi
Andrea M. Aguirre
David Wylensek
Amy C. Coates
Susan A. V. Jennings
Functional Microbiome Research Group, Institute of Medical Microbiology, Rheinisch-Westfälische Technische Hochschule Aachen University Hospital
Atscharah Panyot
Alina Viehof
Matthias A. Schmitz
Institute of Medical Microbiology, Rheinisch-Westfälische Technische Hochschule Aachen University Hospital
Maximilian Stuhrmann
Evelyn C. Deis
Kevin Bisdorf
Maria D. Chiotelli
Artur Lissin
Isabel Schober
Julius Witte
Thorsten Cramer
Thomas Riedel
Marie Wende
Katrin A. Winter
Lena Amend
Alessandra Riva
Stefanie Trinh
Laura Mitchell
Jonathan Hartman
David Berry
Jochen Seitz
Lukas C. Bossert
Marianne Grognot
Thorsten Allers
Till Strowig
Michael Pester
Birte Abt
Lorenz C. Reimer
Jörg Overmann
Thomas Clavel
Functional Microbiome Research Group, Institute of Medical Microbiology, Rheinisch-Westfälische Technische Hochschule Aachen University Hospital