Heteromultivalent Nanogels as Highly Potent Inhibitors of <i>Pseudomonas Aeruginosa</i>

Abstract

Abstract The increasing prevalence of microbial resistance requires new antibacterial concepts for selective targeting and killing of pathogenic bacteria. Here, we report the synthesis of a heteromultivalent nanogel system against Pseudomonas aeruginosa ( P. aeruginosa ). These nanogels are based on biocompatible polyglycerols and functionalized with sugar ligands fucose (Fuc) or galactose (Gal) for P. aeruginosa targeting. With a further modification of these nanogels with BMAP‐18 short chain peptides (GRFKRFRKKFKKLFKKLS), we have achieved &gt; 99.99% inactivation of planktonic and &gt; 99.9% inactivation of biofilm‐coated P. aeruginosa within 12 h of treatment. Additionally, the system demonstrates broad‐spectrum antimicrobial potential, effectively inhibiting Escherichia coli ( E. coli ) and Methicillin‐resistant Staphylococcus aureus (MRSA). This modular design offers a promising strategy for the development of next‐generation antimicrobial therapies targeting biofilm‐associated infections and MDR bacteria.