Genomics of Neotropical biodiversity indicators: Two butterfly radiations with rampant chromosomal rearrangements and hybridization

E Eva S. M. van der Heijden (Tree of Life Programme, Wellcome Sanger Institute) K Karin Näsvall (Tree of Life Programme, Wellcome Sanger Institute) F Fernando A. Seixas (Tree of Life Programme, Wellcome Sanger Institute) C Carlos Eduardo Beserra Nobre (Graduate Program in Animal Biology, Federal University of Pernambuco) A Artur Campos D. Maia (Graduate Program in Animal Biology, Federal University of Pernambuco) P Patricio Salazar-Carrión (Tree of Life Programme, Wellcome Sanger Institute) J Jonah M. Walker (Tree of Life Programme, Wellcome Sanger Institute) D Daiane Szczerbowski (Institute of Organic Chemistry, Technische Universität Braunschweig) S Stefan Schulz I Ian A. Warren (Department of Zoology, University of Cambridge) K Kimberly Gabriela Gavilanes Córdova (Universidad Regional Amazónica Ikiam) M María José Sánchez-Carvajal (Universidad Regional Amazónica Ikiam) F Franz Chandi (Universidad Regional Amazónica Ikiam) A Alex P. Arias-Cruz (Universidad Regional Amazónica Ikiam) N Nicol Rueda-M (Tree of Life Programme, Wellcome Sanger Institute) C Camilo Salazar (Biology Program, Faculty of Natural Sciences, Universidad del Rosario) K Kanchon K. Dasmahapatra (Department of Biology, University of York) S Stephen H. Montgomery (School of Biological Sciences, University of Bristol) M Melanie McClure (Laboratoire Écologie, Évolution, Interactions des Systèmes Amazoniens, Université de Guyane, CNRS, Institut Français de Recherche pour l’Exploitation de la Mer) D Dominic E. Absolon (Tree of Life Programme, Wellcome Sanger Institute) T Thomas C. Mathers (Tree of Life Programme, Wellcome Sanger Institute) C Camilla A. Santos (Tree of Life Programme, Wellcome Sanger Institute) S Shane McCarthy (Tree of Life Programme, Wellcome Sanger Institute) J Jonathan M. D. Wood (Tree of Life Programme, Wellcome Sanger Institute) G Gerardo Lamas (Museo de Historia Natural, Universidad Nacional Mayor de San Marcos) C Caroline Bacquet (Universidad Regional Amazónica Ikiam) A André Victor Lucci Freitas (Laboratório de Ecologia e Sistemática de Borboletas, Instituto de Biologia, Universidade Estadual de Campinas) K Keith R. Willmott (McGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of Florida) C Chris D. Jiggins (Department of Zoology, University of Cambridge) M Marianne Elias (Smithsonian Tropical Research Institute) J Joana I. Meier (Tree of Life Programme, Wellcome Sanger Institute)

Abstract

A central question in evolutionary biology is what drives the diversification of lineages. Rapid, recent radiations are ideal systems for this question because they still show key morphological and ecological adaptations associated with speciation. While most research on recent radiations focuses on those occurring in insular environments, less attention has been given to continental radiations with complex species interactions. Here, we study the drivers of continental radiations of Melinaea and Mechanitis butterflies (Nymphalidae: Ithomiini), which have rapidly radiated in the continental Neotropics. They are classical models for Amazonian biogeography and color pattern mimicry and have been proposed as biodiversity indicators. We generated reference genomes for five species of each genus and whole-genome resequencing data of most species and subspecies covering a wide geographic range to assess phylogeographic relationships, hybridization patterns, and chromosomal rearrangements. Our data help resolve the classification of these taxonomically challenging butterflies and reveal very high diversification rates. We find rampant evidence of historical hybridization and putative hybrid species in both radiations, which may have facilitated their rapid diversification by enriching the genetic diversity. Moreover, we identified dozens of chromosomal fusions and fissions between congeneric species that have likely expedited reproductive isolation. We conclude that interactions between geography, hybridization and chromosomal rearrangements have contributed to these rapid radiations in the highly diverse Neotropical region. We hypothesize that rapid radiations may be spurred if repeated periods of geographic isolation are combined with lineage-specific rapid accumulation of incompatibilities, followed by secondary contact with some gene exchange.

Article Details

Volume / Issue Vol. 122, Issue 31
Published August 05, 2025
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (31)

E

Eva S. M. van der Heijden

Tree of Life Programme, Wellcome Sanger Institute

K

Karin Näsvall

Tree of Life Programme, Wellcome Sanger Institute

F

Fernando A. Seixas

Tree of Life Programme, Wellcome Sanger Institute

C

Carlos Eduardo Beserra Nobre

Graduate Program in Animal Biology, Federal University of Pernambuco

A

Artur Campos D. Maia

Graduate Program in Animal Biology, Federal University of Pernambuco

P

Patricio Salazar-Carrión

Tree of Life Programme, Wellcome Sanger Institute

J

Jonah M. Walker

Tree of Life Programme, Wellcome Sanger Institute

D

Daiane Szczerbowski

Institute of Organic Chemistry, Technische Universität Braunschweig

S

Stefan Schulz

I

Ian A. Warren

Department of Zoology, University of Cambridge

K

Kimberly Gabriela Gavilanes Córdova

Universidad Regional Amazónica Ikiam

M

María José Sánchez-Carvajal

Universidad Regional Amazónica Ikiam

F

Franz Chandi

Universidad Regional Amazónica Ikiam

A

Alex P. Arias-Cruz

Universidad Regional Amazónica Ikiam

N

Nicol Rueda-M

Tree of Life Programme, Wellcome Sanger Institute

C

Camilo Salazar

Biology Program, Faculty of Natural Sciences, Universidad del Rosario

K

Kanchon K. Dasmahapatra

Department of Biology, University of York

S

Stephen H. Montgomery

School of Biological Sciences, University of Bristol

M

Melanie McClure

Laboratoire Écologie, Évolution, Interactions des Systèmes Amazoniens, Université de Guyane, CNRS, Institut Français de Recherche pour l’Exploitation de la Mer

D

Dominic E. Absolon

Tree of Life Programme, Wellcome Sanger Institute

T

Thomas C. Mathers

Tree of Life Programme, Wellcome Sanger Institute

C

Camilla A. Santos

Tree of Life Programme, Wellcome Sanger Institute

S

Shane McCarthy

Tree of Life Programme, Wellcome Sanger Institute

J

Jonathan M. D. Wood

Tree of Life Programme, Wellcome Sanger Institute

G

Gerardo Lamas

Museo de Historia Natural, Universidad Nacional Mayor de San Marcos

C

Caroline Bacquet

Universidad Regional Amazónica Ikiam

A

André Victor Lucci Freitas

Laboratório de Ecologia e Sistemática de Borboletas, Instituto de Biologia, Universidade Estadual de Campinas

K

Keith R. Willmott

McGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of Florida

C

Chris D. Jiggins

Department of Zoology, University of Cambridge

M

Marianne Elias

Smithsonian Tropical Research Institute

J

Joana I. Meier

Tree of Life Programme, Wellcome Sanger Institute