Genome sequencing of a novel Verticillium dahliae strain (huangweibingjun)

Abstract

Abstract Verticillium dahliae is a soilborne pathogenic fungus that causes vascular discoloration and wilting in a broad spectrum of plant hosts, affecting about 400 species, such as cotton, potatoes, watermelon, cucumber, spinach, etc. In 2021, V. dahliae was estimated to cause about 15–20% reduction in cotton in China. Here, we report the genome sequencing of a novel strain named huangweibingjun, isolated from diseased cotton roots in the Henan province of China. The huangweibingjun genome consists of a total size of 35.84 Mb, GC content of 59.835%, and harbors six chromosomes (scaffold7561, scaffold7329, scaffold7795, scaffold5491, scaffold5473, and scaffold4511). The genome architecture showed a high diversity of cell wall-degrading secretory proteins that might influence the pathogenicity of the fungal strain. Moreover, preliminary metabolic pathway prediction showed that this novel strain synthesizes polyketide, terpenoids, shikimic acid-derived compounds and could also be aflatoxigenic. Consistent with other pathogenic microbes, the huangweibingjun genome comprises several virulent-associated genes. This genome assembly lays the foundation for further investigation of the pathogenicity of huangweibingjun.