Genetic recording of pancreatic beta cell proliferation
Abstract
Pancreatic beta cell generation and proliferation primarily rely on the self renewal of preexisting beta cells, making precise quantification of beta cell proliferation essential for understanding pancreatic homeostasis and pathogenesis. However, previous methods had limitations and were unable to trace beta cell proliferation in vivo over extended periods. Here, we developed beta cell ProTracer, a genetic system that uses dual recombinase technology to enable continuous and cell type-specific recording of beta cell proliferation in mice. The beta cell ProTracer system allows for the quantification of the proliferation rate of adult pancreatic beta cells during homeostasis and in response to injury or drug treatment. Clonal analysis of the proliferated beta cell population reveals a uniform proliferative capacity among these cells. Our findings enhance the understanding of beta cell proliferation dynamics in homeostasis, repair, and regeneration.
Article Details
Journal Info
Proceedings of the National Academy of Sciences
National Academy of Sciences
Authors (10)
Huan Zhao
Hui Chen
Zhixin Kang
Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences
Xiuzhen Huang
Chinese Academy of Sciences Center for Excellence in Molecuar Cell Science-Chinese University of Hong kong (CAS CEMCS-CUHK) Joint Laboratories, New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences
Jingting Zhu
Chinese Academy of Sciences Center for Excellence in Molecular Cell Science-The Chinese University of Hong Kong Joint Laboratories, New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences
Zixin Liu
Tsinghua University , , ,
Xiuxiu Liu
Chinese Academy of Sciences Center for Excellence in Molecular Cell Science-The Chinese University of Hong Kong Joint Laboratories, New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences
Ximeng Han
Chinese Academy of Sciences Center for Excellence in Molecular Cell Science-The Chinese University of Hong Kong Joint Laboratories, New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences
Jie Lu
Bin Zhou