Functional connectomics spanning multiple areas of mouse visual cortex
Abstract
Abstract Understanding the brain requires understanding neurons’ functional responses to the circuit architecture shaping them. Here we introduce the MICrONS functional connectomics dataset with dense calcium imaging of around 75,000 neurons in primary visual cortex (VISp) and higher visual areas (VISrl, VISal and VISlm) in an awake mouse that is viewing natural and synthetic stimuli. These data are co-registered with an electron microscopy reconstruction containing more than 200,000 cells and 0.5 billion synapses. Proofreading of a subset of neurons yielded reconstructions that include complete dendritic trees as well the local and inter-areal axonal projections that map up to thousands of cell-to-cell connections per neuron. Released as an open-access resource, this dataset includes the tools for data retrieval and analysis 1,2 . Accompanying studies describe its use for comprehensive characterization of cell types 3–6 , a synaptic level connectivity diagram of a cortical column 4 , and uncovering cell-type-specific inhibitory connectivity that can be linked to gene expression data 4,7 . Functionally, we identify new computational principles of how information is integrated across visual space 8 , characterize novel types of neuronal invariances 9 and bring structure and function together to uncover a general principle for connectivity between excitatory neurons within and across areas 10,11 .
Article Details
Authors (96)
J. Alexander Bae
Mahaly Baptiste
Maya R. Baptiste
Caitlyn A. Bishop
Agnes L. Bodor
Derrick Brittain
Victoria Brooks
JoAnn Buchanan
Daniel J. Bumbarger
Manuel A. Castro
Brendan Celii
Erick Cobos
Forrest Collman
Nuno Maçarico da Costa
Bethanny Danskin
Sven Dorkenwald
Leila Elabbady
Paul G. Fahey
Tim Fliss
Emmanouil Froudarakis
Jay Gager
Clare Gamlin
William Gray-Roncal
Akhilesh Halageri
James Hebditch
Zhen Jia
Emily Joyce
Justin Ellis-Joyce
Chris Jordan
Daniel Kapner
Nico Kemnitz
Sam Kinn
Lindsey M. Kitchell
Selden Koolman
Kai Kuehner
Kisuk Lee
Kai Li
Ran Lu
Thomas Macrina
Gayathri Mahalingam
Jordan Matelsky
Sarah McReynolds
Elanine Miranda
Eric Mitchell
Shanka Subhra Mondal
Merlin Moore
Shang Mu
Taliah Muhammad
Barak Nehoran
Erika Neace
Oluwaseun Ogedengbe
Christos Papadopoulos
Stelios Papadopoulos
Saumil Patel
Guadalupe Jovita Yasmin Perez Vega
Xaq Pitkow
Sergiy Popovych
Anthony Ramos
R. Clay Reid
Jacob Reimer
Patricia K. Rivlin
Victoria Rose
Zachary M. Sauter
Casey M. Schneider-Mizell
H. Sebastian Seung
Ben Silverman
William Silversmith
Amy Sterling
Fabian H. Sinz
Cameron L. Smith
Rachael Swanstrom
Shelby Suckow
Marc Takeno
Zheng H. Tan
Andreas S. Tolias
Russel Torres
Nicholas L. Turner
Edgar Y. Walker
Tianyu Wang
Adrian Wanner
Brock A. Wester
Grace Williams
Sarah Williams
Kyle Willie
Ryan Willie
William Wong
Jingpeng Wu
Chris Xu
Runzhe Yang
Dimitri Yatsenko
Fei Ye
Wenjing Yin
Rob Young
Szi-chieh Yu
Daniel Xenes
Chi Zhang