Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets

M Matthew Penner (Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.) O Oskar James Klein (Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.) M Maximilian Gantz (Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.) F Friederike E. H. Nintzel (Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.) A Anne-Cathrin Prowald (Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.) S Sally Boss (Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.) P Paul Barker (Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.) P Paul Dupree F Florian Hollfelder (Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.)

Article Details

Volume / Issue Vol. 147, Issue 13
Published April 02, 2025
Pages 10903-10915
ISSN 0002-7863
Publisher American Chemical Society

Journal Info

Journal of the American Chemical Society

American Chemical Society

ISSN: 0002-7863 Physical Sciences

Authors (9)

M

Matthew Penner

Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.

O

Oskar James Klein

Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.

M

Maximilian Gantz

Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.

F

Friederike E. H. Nintzel

Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.

A

Anne-Cathrin Prowald

Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.

S

Sally Boss

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.

P

Paul Barker

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.

P

Paul Dupree

F

Florian Hollfelder

Department of Biochemistry, University of Cambridge, 80 Tennis Court Rd, Cambridge CB2 1GA, U.K.