Erasable Fluorescence Imaging Technology Enables Continuous Tracking for Identical Living Cells
Abstract
Abstract Subtle molecular events may trigger a cascade of “butterfly effects” to reverse cell fate, so dynamic tracking of relevant markers can provide crucial clues to decipher the unknown. However, existing analytical techniques are still at static‐analysis level, and cutting‐edge fluorescence imaging is limited by signal interference at adjacent time, failing to realize continuous observation for identical live cells. Herein, we develop a new temperature‐controlled imaging technology, where melamine‐mediated reversible DNA self‐assembly drives the fluorescence illuminating and extinguishing at any time, achieving repeated erasable imaging for identical living cells. This method can track cell behaviors by taking dynamic monitoring of cell differentiation as an example, and timely fluorescence erasing guarantees authenticity for imaging results and minimizes interference with cellular activities. Thanks to high feasibility in fundamental experimental scenarios, this method hopefully provides a powerful preliminary screening tool for exploring new biological mechanisms in cell interactions, developmental transformations, downstream response inquiry, and so forth.
Article Details
Authors (9)
Yanan Peng
Qiumei Pu
Liangqing Lu
NHC Key Laboratory of Tropical Disease Control School of Tropical Medicine & The Second Affiliated Hospital International Center for Aging and Cancer Key Laboratory of Emergency and Trauma of Ministry of Education The First Affiliated Hospital Hainan Academy of Medical Sciences Hainan Medical University Haikou 571199 China
Qionglin Zhou
NHC Key Laboratory of Tropical Disease Control School of Tropical Medicine & The Second Affiliated Hospital International Center for Aging and Cancer Key Laboratory of Emergency and Trauma of Ministry of Education The First Affiliated Hospital Hainan Academy of Medical Sciences Hainan Medical University Haikou 571199 China
Xinxin Xiao
NHC Key Laboratory of Tropical Disease Control School of Tropical Medicine & The Second Affiliated Hospital International Center for Aging and Cancer Key Laboratory of Emergency and Trauma of Ministry of Education The First Affiliated Hospital Hainan Academy of Medical Sciences Hainan Medical University Haikou 571199 China
Xiangde Lai
NHC Key Laboratory of Tropical Disease Control School of Tropical Medicine & The Second Affiliated Hospital International Center for Aging and Cancer Key Laboratory of Emergency and Trauma of Ministry of Education The First Affiliated Hospital Hainan Academy of Medical Sciences Hainan Medical University Haikou 571199 China
Xuan Zhao
Bin Qiao
Qiang Wu
Jiangsu Cancer Hospital Nanjing China