Epigenetic alterations in type 1 diabetes and their association with poor glycemic control: the SED1-EPI substudy
Abstract
Abstract Type 1 diabetes (T1D) is an autoimmune disease characterized by β-cell destruction and insulin deficiency. Circulating microRNAs (miRNAs) are promising biomarkers for disease monitoring and complication risk assessment. This study aimed to describe the epigenetic profile in a multicenter Spanish T1D cohort and its association with clinical variables. We analyzed plasma samples from 125 participants (76 T1D, 49 controls) recruited across nine Spanish hospitals. Eight candidate miRNAs from a prior Asturias cohort study were quantified using RT-PCR. Associations with clinical and biochemical parameters were explored, and enrichment analysis was performed to investigate affected biological pathways. Of the miRNAs analyzed, only hsa-miR-200a-3p was significantly overexpressed in T1D versus controls ( p = 0.035). In T1D patients, hsa-miR-200a-3p, hsa-miR-1-3p, and hsa-miR-340-5p showed positive correlations with HbA1c, while hsa-miR-224-5p correlated with body mass index (BMI). Poor glycemic control (HbA1c ≥ 7.5%) was associated with higher expression of hsa-miR-1-3p, hsa-miR-200a-3p, and hsa-miR-340-5p. No significant expression differences were found according to comorbidity or hypertension status. hsa-miR-200a-3p emerges as a potential biomarker for β-cell stress in long-standing T1D, while hsa-miR-1-3p and hsa-miR-340-5p may reflect glycemic control status and vascular risk. These findings support further evaluation of these miRNAs in larger, clinically diverse T1D cohorts.
Article Details
Authors (82)
Ana Victoria García
Carmen Lambert
Elsa Villa-Fernández
Miguel García-Villarino
Isabel Serrano-Olmedo
Elsa Fernández Rubio
Ana Megia Colet
Miguel Brito-Sanfiel
Francisca Payeras Mas
Estefanía Santos
M. Ángeles Martínez de Salinas Santamaría
Fernando Gómez-Peralta
Edelmiro Menéndez-Torre
Pedro Pujante
Francisco Tinahones Madueño
Florentino Carral San Laureano
Martín López de la Torre
Alberto Moreno Carazo
Javier Acha Pérez Hospital
Orosia Bandrés Nivela
Lluís Masmiquel Comas
Ignacio Llorente Gómez
Juan Angel Hernández Bayo
Coral Montalbán
Daniel de Luis
Gonzalo Díaz Soto
Antonio López-Guzmán
Luz Ma López Jiménez
Visitacion Alvarez
Benito Blanco Samper
Ana Chico Ballesteros
Belen Dalama Gómez
Ignacio Conget
Manuel Pérez Maraver
Berta Soldevila
Ismael Capel Flores
Marta Hernández García
Wifredo Ricart
Elisenda Climent Biescas
Francisco Javier Ampudia Blasco
Antonio Hernández Mijares
Carlos Sánchez Juan
Antonio Picó
José Ramón Domínguez Escribano
Carmiña Fajardo Montañana
Teresa Pedro
Pablo Abellán
Paolo Rossetti
Computational Biology
Francisco M. Morales-Pérez
Fidel Enciso
Alfonso Soto González
Diego Bellido Guerrero
Reyes Luna Cano
José Manuel García López
Víctor M. Andía Melero
José Alfonso Arranz Martín
Sharona Azriel Mira
Marta Botella Serrano
Alfonso Calle Pascual
Francisco Javier del Cañizo Gómez
Manuel Ángel Gargallo Fernández
Fátima Illán
Antonio M. Hernández Martínez
Lluis Forga Llenas
Clara Rosario Fuentes Gómez
Amelia Oleaga
Juan Pedro López-Siguero
Ana Lucía Gómez-Gila
Alfonso María Lechuga Sancho
Marta Ferrer Lozano
Isolina Riaño Galán
María Caimari
Roque Cardona
María Clemente León
Gemma Carreras González
Francisco Javier Arroyo Diez
Paloma Cabanas Rodríguez
Belén Roldán
Noemí González Pérez de Villar
Purificación Ros Pérez
Itxaso Rica
Ignacio Diez López