Enhanced blood–brain barrier opening induced by focused ultrasound combined with microbubble clusters trapped by an acoustic vortex tweezer
Abstract
Focused ultrasound (FUS) combined with microbubbles (MBs) represents a noninvasive, targeted approach for transiently opening the blood–brain barrier (BBB). While higher MB concentrations can enhance BBB permeability, they also raise the risk of adverse effects such as erythrocyte extravasation. In this study, acoustic vortex (AV) manipulation of MBs was integrated with FUS-mediated BBB opening. The results demonstrated that dispersed MBs could be effectively clustered by an AV tweezer in multi-well plates. Compared with FUS applied to dispersed MBs, the combination of AV, FUS, and MBs required lower acoustic pressure, achieved higher BBB opening efficiency, and elicited significantly stronger stable cavitation. In addition, both pore density and size on cell membranes were greater in the AV + FUS + MB group relative to the FUS + MB group. Importantly, temperature elevation was minimal and cell viability was preserved. These findings suggest that combining FUS with MB clusters trapped by an AV tweezer offers a promising and efficient strategy for BBB opening.
Article Details
Journal Info
Journal of Applied Physics
American Institute of Physics
Authors (10)
Shifang Guo
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University 1 , Xi'an 710049,
Wanlin Jia
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University 1 , Xi'an 710049,
Xinru Hu
State Key Laboratory of Bioreactor Engineering Shanghai Key Laboratory of Chemical Biology School of Pharmacy East China University of Science and Technology 130 Meilong Road Shanghai 200237 China
Yan Li
Zhen Ya
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University 1 , Xi'an 710049,
Chaoyu Wang
Department of Chemistry
Pengfei Xu
Diya Wang
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University 1 , Xi'an 710049,
Lei Zhang
Mingxi Wan
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University 1 , Xi'an 710049,