Nature Communications

Engineered alcohol oxidases catalyse transesterification in aqueous media without competing hydrolysis

B Bin Wu Y Yunjian Ma C Chenhao Feng L Limei Ren C Chiara Domestici Y Yutong Wang T Thomas Hilberath U Ulf Hanefeld E Evgeny A. Pidko (Inorganic Systems Engineering Group, Department of Chemical Engineering, Faculty of Applied Sciences, Delft University of Technology, Van der Maasweg 9, Delft 2629 HZ, The Netherlands) F Frank Hollmann Y Yonghua Wang

Abstract

Abstract Transesterification reactions are fundamental transformations in organic chemistry, yet performing them in aqueous media is challenging because of the competing hydrolysis reaction. In this study, we describe a mutant of alcohol oxidase from Phanerochaete chrysosporium ( Pc AOx-VPN) that also exhibits transesterification activity. Moreover, Pc AOx-VPN displays no detectable hydrolytic activity, owing to its hydrophobic active site, which effectively excludes water. These characteristics make Pc AOx-VPN a promising catalyst for transesterification reactions in aqueous media, a context that is typically compromised by competing hydrolysis.

Download PDF View Original
APA MLA Chicago BibTeX RIS

Article Details

Volume / Issue Vol. 17, Issue 1
Published January 30, 2026
ISSN 2041-1723
Publisher Nature Portfolio

Journal Info

Nature Communications

Nature Portfolio
ISSN: 2041-1723 Open Access Life Sciences

Authors (11)

B

Bin Wu

Y

Yunjian Ma

C

Chenhao Feng

L

Limei Ren

C

Chiara Domestici

Y

Yutong Wang

T

Thomas Hilberath

U

Ulf Hanefeld

E

Evgeny A. Pidko

Inorganic Systems Engineering Group, Department of Chemical Engineering, Faculty of Applied Sciences, Delft University of Technology, Van der Maasweg 9, Delft 2629 HZ, The Netherlands

F

Frank Hollmann

Y

Yonghua Wang

Related Articles from this Journal

Synthesis of adaptive 15N-nitrosyl-Co7 nanocluster for electrocatalytic C–H functionalization

Chao Wu, Xu Zhang et al. Aug 2026 10.1038/s41467-026-76516-1

Pan-Ebolavirus nanoparticle vaccine provides protection in rodents from lethal infection by Zaire and Sudan viruses

Connor Weidle, Natalie Brunette et al. Aug 2026 10.1038/s41467-026-76114-1

Multi-population GWAS meta-analysis identifies bladder cancer susceptibility loci and highlights genetic regulation of smoking-related risk

Ludmila Prokunina-Olsson, Oscar Florez-Vargas et al. Aug 2026 10.1038/s41467-026-76157-4

Genome-wide annotation of human multi-nucleotide variants reveals widespread functional differences from single nucleotide variants

Weiwei Jin, Wen Cao et al. Aug 2026 10.1038/s41467-026-76470-y

Allele-specific chromatin architecture shapes imprinted domains and coordinates a distal enhancer and antisense transcription at the mouse Mest-Copg2 domain

Bongmin Bae, Katherine Gu et al. Aug 2026 10.1038/s41467-026-76506-3