Emergence of genetic sex determination in an environmentally sex-determined animal

W Wen Wei (Biodesign Center for Mechanisms of Evolution, Arizona State University) M Man Lin (Biodesign Center for Mechanisms of Evolution, Arizona State University) T Trent Bishop (Biodesign Center for Mechanisms of Evolution, Arizona State University) R Ryan Shahriari (Biodesign Center for Mechanisms of Evolution, Arizona State University) Y Yue Hao L Logan Graham (Biodesign Center for Mechanisms of Evolution, Arizona State University) B Bret L. Coggins (Department of Biological Sciences, Notre Dame University) H Hannah Jee (Biodesign Center for Mechanisms of Evolution, Arizona State University) E Emma Fonseca (Biodesign Center for Mechanisms of Evolution, Arizona State University) S Swatantra Neupane (Division of Biological Sciences, University of Missouri) L Li Wang (The Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital Zhengzhou China) S Sen Xu (Division of Biological Sciences, University of Missouri) E Erin Kim (Biodesign Center for Mechanisms of Evolution, Arizona State University) M Michael Lynch (Biodesign Center for Mechanisms of Evolution) Z Zhiqiang Ye (Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, School of Life Sciences, Central China Normal University)

Abstract

The evolution of genetic sex determination (GSD) from environmental sex determination (ESD) remains a fundamental issue in evolutionary biology. However, the mechanisms driving the transition, particularly in its earliest stages, are largely unknown. Here, we report a genetic variant in the ESD species, Daphnia pulex , in which structural variations on chromosome I have suppressed recombination. This suppression appears to have facilitated the accumulation of divergent features of two chromosome I haplotypes, resulting in differentiation of the linked sex-determining gene Dfh . This process has led to the emergence of a mixed-mating system, in which sex determination is jointly regulated by genetic and environmental factors, with ongoing divergence in chromosomal structure continuing to develop between the two haplotypes at the population level. These findings suggest that processes analogous to early sex-chromosome evolution are occurring in this ESD species, offering insights into the molecular mechanisms underlying the emergence of GSD.

Article Details

Volume / Issue Vol. 123, Issue 17
Published April 28, 2026
ISSN 0027-8424
Publisher National Academy of Sciences

Authors (15)

W

Wen Wei

Biodesign Center for Mechanisms of Evolution, Arizona State University

M

Man Lin

Biodesign Center for Mechanisms of Evolution, Arizona State University

T

Trent Bishop

Biodesign Center for Mechanisms of Evolution, Arizona State University

R

Ryan Shahriari

Biodesign Center for Mechanisms of Evolution, Arizona State University

Y

Yue Hao

L

Logan Graham

Biodesign Center for Mechanisms of Evolution, Arizona State University

B

Bret L. Coggins

Department of Biological Sciences, Notre Dame University

H

Hannah Jee

Biodesign Center for Mechanisms of Evolution, Arizona State University

E

Emma Fonseca

Biodesign Center for Mechanisms of Evolution, Arizona State University

S

Swatantra Neupane

Division of Biological Sciences, University of Missouri

L

Li Wang

The Affiliated Cancer Hospital of Zhengzhou University and Henan Cancer Hospital Zhengzhou China

S

Sen Xu

Division of Biological Sciences, University of Missouri

E

Erin Kim

Biodesign Center for Mechanisms of Evolution, Arizona State University

M

Michael Lynch

Biodesign Center for Mechanisms of Evolution

Z

Zhiqiang Ye

Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, School of Life Sciences, Central China Normal University